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anti-AAV5 (intact particle) mouse monoclonal, ADK5b, lyophilized, purified

Specifically detects intact virus particles, both empty and full capsids. ADK5b recognizes a conformational epitope of assembled capsids, not present in denatured and native unassembled capsid proteins. No cross-reaction with other AAV serotypes. The antibody is not suitable for Immunoblotting.
Cat. No.: 610149
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€323.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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anti-AAV5 (intact particle) mouse monoclonal, ADK5b

lyophilized, purified, sample 10 µg
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Excl. 19% Tax, excl. Shipping Cost

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b kappa
Clone ADK5b
Immunogen adeno-associated virus type 5 (AAV5) capsid proteins and virus particles
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity AAV5 (found in human and monkey)

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:20
Immunohistochemistry (IHC) - frozen assay dependent
Immunohistochemistry (IHC) - paraffin assay dependent (microwave treatment recommended)
Immunoprecipitation (IP) 1:5
Neutralization Assay assay dependent

Background

Details

For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK5b specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids, not present in denatured capsid proteins and native but unassembled capsid proteins. The antibody cannot be used for immunoblotting.

Limited Use Label License: Research Use Only
Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

Reference

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