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anti-AAV8 (intact particle) mouse monoclonal, ADK8, supernatant Zoom

anti-AAV8 (intact particle) mouse monoclonal, ADK8, supernatant

anti-AAV8 (intact particle) mouse monoclonal, ADK8, supernatant

For characterization of different stages of infection and very useful for analysis of the AAV assembly process. ADK8 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Not for use in immunoblotting.
Cat. No.: 651160
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€229.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a kappa
Clone ADK8
Immunogen recombinantly adeno-associated virus type 8 (AAV-8)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity AAV8 (no cross-reaction with other AAV types in IF. weak cross-reactivity with AAV1, 3, 7 and 10 in dot blot (Mietzsch et al., 2014))

Applications

Dot Blot assay dependent (non-denaturing conditions)
ELISA assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Immunoprecipitation (IP) assay dependent
Neutralization Assay assay dependent (dialyze to remove the sodium azid)

Background

Details

For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK8 specifically reacts with intact adeno-associated virus particles, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Predicted binding site: residues 586-LQQQNT-591 (Gurda et al., 2012). The antibody cannot be used for immunoblotting using denaturing conditions.

Reference

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