Dot blot with different AAV serotypes and mouse monoclonal anti-AAV8 antibody, clone ADK8 (Courtesy of Regina Heilbronn, Charité Universitätsmedizin Berlin, Mietzsch et al. Hum Gene Ther. 2014 Mar 1; 25(3):212-222)

anti-AAV8 (intact particle) mouse monoclonal, ADK8, supernatant

For characterization of different stages of infection and very useful for analysis of the AAV assembly process. ADK8 specifically reacts with AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Not for use in immunoblotting.

The ADK8 antibody is used as capture and detection antibody in the AAV8 Titration ELISA (PRAAV8), the AAVrh10 Titration ELISA (PRAAV10) and the AAV8 Xpress ELISA (PRAAV8XP).

Cat. No.: 651160
Quantity: 5 mL

Delivery Time: usually 1-7 working days

$303.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Tseng, Y.-S. et al. Generation and characterization of anti-Adeno-associated virus serotype 8 (AAV8) and anti-AAV9 monoclonal antibodies. J. Virol. Methods 236, 105-110 (2016).

AAV3
AAV8

dot blot
neutralization

Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1-12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212-222 (2014).

AAV1
AAV3
AAV7
AAV8
AAVrh10

dot blot

Gurda, B. L. et al. Mapping a Neutralizing Epitope onto the Capsid of Adeno-Associated Virus Serotype 8. J. Virol. 86, 7739-7751 (2012).

AAV8

ELISA
dot blot
ICC-IF
neutralization
epitope mapping

Raupp, C. et al. The Threefold Protrusions of Adeno-Associated Virus Type 8 Are Involved in Cell Surface Targeting as Well as Postattachment Processing. J. Virol. 86, 9396-9408 (2012).

AAV8

neutralization

Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686-12697 (2011).

AAV3
AAV8
AAV9

ELISA
dot blot
ICC-IF

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Specifications

Datasheet

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
610160	anti-AAV8 (intact particle) mouse monoclonal, ADK8	lyophilized, purified	50 µg	$427.00 Excl. VAT, excl. Shipping Cost
610160S	anti-AAV8 (intact particle) mouse monoclonal, ADK8	lyophilized, purified, sample	10 µg	$128.00 Excl. VAT, excl. Shipping Cost
615160	anti-AAV8 (intact particle) mouse monoclonal, ADK8	Biotin Conjugate	750 µL	$280.00 Excl. VAT, excl. Shipping Cost

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG2a kappa
Clone	ADK8
Immunogen	AAV8 capsids
Purification	hybridoma cell culture supernatant
Conjugate	unconjugated
Formulation	contains 0.09% sodium azide
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
Intended use	Research use only
Tested species reactivity	AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74, Anc80

Applications

Tested applications	Tested dilutions
Dot Blot	assay dependent (non-denaturing conditions)
ELISA	assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	assay dependent
Immunoprecipitation (IP)	assay dependent
Neutralization Assay	assay dependent

Background

Details

For characterization of different stages of infection and very useful for the analysis of the AAV assembly process. ADK8 specifically reacts with AAV1, AAV3, AAV7, AAV8, AAVrh10, AAVrh74 and Anc80, empty and full capsids. Recognizes a conformational epitope of assembled capsids. Predicted binding site: residues 586-LQQQNT-591 (Gurda et al., 2012). The antibody cannot be used for immunoblotting using denaturing conditions.
The antibody is also useful for neutralizing experiments.

Gurda BL, et al. Mapping a neutralizing epitope onto the capsid of adeno-associated virus serotype 8. J Virology 86, 7739-7751 (2012).

Limited Use Label License: Research Use Only
Product is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivatives which are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.

Reference

Publication

Species

Application

Tseng, Y.-S. et al. Generation and characterization of anti-Adeno-associated virus serotype 8 (AAV8) and anti-AAV9 monoclonal antibodies. J. Virol. Methods 236, 105-110 (2016).

AAV3
AAV8

dot blot
neutralization

Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1-12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212-222 (2014).

AAV1
AAV3
AAV7
AAV8
AAVrh10

dot blot

Gurda, B. L. et al. Mapping a Neutralizing Epitope onto the Capsid of Adeno-Associated Virus Serotype 8. J. Virol. 86, 7739-7751 (2012).

AAV8

ELISA
dot blot
ICC-IF
neutralization
epitope mapping

Raupp, C. et al. The Threefold Protrusions of Adeno-Associated Virus Type 8 Are Involved in Cell Surface Targeting as Well as Postattachment Processing. J. Virol. 86, 9396-9408 (2012).

AAV8

neutralization

Sonntag, F. et al. The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes. J. Virol. 85, 12686-12697 (2011).

AAV3
AAV8
AAV9

ELISA
dot blot
ICC-IF

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PRAAV8), the AAVrh10 Titration ELISA (PRAAV10) and the AAV8 Xpress ELISA (PRAAV8XP).

anti-AAV8 (intact particle) mouse monoclonal, ADK8, supernatant

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