anti-acidic Hair Keratin K34 guinea pig polyclonal, serum
- Guinea pig polyclonal
- Suitable for IHC and WB
- Reacts with human and mouse
|Storage||Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles|
|Intended use||Research use only|
|Immunogen||Synthetic peptide of human acid ic hair (trichocytic) keratin K34 (formerly also designated keratin hHa4; GPCGTSQKGCCN), coupled to KLH|
|Formulation||Contains 0.09% sodium azide and 0.5% BSA|
|UniprotID||O76011 (Human), Q9D646 (Mouse)|
|Synonym||Keratin, type I cuticular Ha4, Hair keratin, type I Ha4, Keratin-34, K34, KRT34, HHA4, HKA4, KRTHA4|
|Note||Centrifuge prior to opening|
|Tested applications||Tested dilutions|
|Immunohistochemistry (IHC) - frozen||1:200 (for enhancement of cortex staining preincubate fixed sections with 0.1% Triton X-100 (in PBS) for 1-5 min prior to first antibody incubation step)|
|Western Blot (WB)||1:2,000|
The antibody stains specifically human hair keratin K34 (hHa4) expressed in the upper hair cortex cells.
Publication Langbein, L., Yoshida, H., Praetzel-Wunder, S., Parry, D. A. & Schweizer, J. The Keratins of the Human Beard Hair Medulla: The Riddle in the Middle. J. Invest. Dermatol. 130, 55–73 (2010).
Application IHC (frozen)
Publication Langbein, L. et al. The catalog of human hair keratins. I. Expression of the nine type I members in the hair follicle. J. Biol. Chem. 274, 19874–84 (1999).
Application WB,IHC (frozen)
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