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human scalp hair (courtesy of L. Langbein) Zoom

human scalp hair (courtesy of L. Langbein)

anti-acidic Hair Keratin K32 guinea pig polyclonal, serum

The antiserum stains specifically human hair keratin K32 (hHa2) expressed in the hair cuticle (Mr 45,000 polypeptide) and tumors derived from.
Cat. No.: GP-hHa2
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€260.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic peptide of human hair (trichocytic) keratin K32 (formerly also designated keratin hHa2; C-VTRTVCVPRTVGM), coupled to KLH
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human (negative with mouse)

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:200 (for enhancement of cortex staining preincubate fixed sections with 0.1 Triton X-100 (in PBS) for 1 – 5 min prior to first antibody incubation step)
Immunohistochemistry (IHC) - paraffin 1:200 (protease treatment and microwave treatment recommended; for enhancement of cortex staining preincubate fixed sections with 0.1 Triton X-100 (in PBS) for 1 – 5 min prior to first antibody incubation step)
Western Blot (WB) 1:2,000

Background

Details

The antiserum stains specifically human hair keratin K32 expressed in the hair cuticle (Mr 45,000 polypeptide) and tumors derived from.

Reference

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