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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | Synthetic peptide of human hair (trichocytic) keratin K33 (formerly also designated keratin hHa3; EKP IGS CVT NPC GPR SR), coupled to KLH |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human, mouse |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:200 (for enhancement of cortex staining preincubate fixed sections with 0.1 Triton X-100 (in PBS) for 1 – 5 min prior to first antibody incubation step) |
| Western Blot (WB) | 1:2,000 |
Background
Details
The antiserum stains specifically human hair keratin K33 expressed starting from the middle hair cortex.
Because of high sequence homology both keratin isoforms K33a (formerly also designated keratin hHa3-I) and K33b (formerly designated keratin hHa3-II) are detected.
Reference
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