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Product description
| Host | guinea pig |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | synthetic peptide of human acidic hair (trichocytic) keratin K38 (formerly also designated keratin hHa8; TTC GPT CGA STT GSR F), coupled to KLH |
| Purification | stabilized antiserum |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide and 0.5% BSA |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human, mouse |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:200 (for enhancement of cortex staining preincubate fixed sec-tions with 0.1 Triton X-100 (in PBS) for 1 – 5 min prior to first antibody incubation step) |
| Western Blot (WB) | 1:2,000 |
Background
Details
The antiserum stains specifically human hair keratin K38 expressed starting from the middle cortex. The staining in the middle cortex appears highly heterogeneous.
Reference
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