Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgM |
Clone | 2G2 |
Immunogen | profilin actin complex from calf thymus |
Purification | hybridoma cell culture supernatant |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 100 (IHC, 50 µl/slide) |
Intended use | Research use only |
Tested species reactivity | bovine, chicken, human, plant, rabbit |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent (IF only after methanol fixation) |
Immunohistochemistry (IHC) - frozen | ready-to-use |
Immunohistochemistry (IHC) - paraffin | ready-to-use (protease treatment recommended) |
Western Blot (WB) | assay dependent |
Background
Details
Specific for skeletal and non-muscle actin isoforms (Mr 43,000). Epitope location: NVPAMYVAVLDSGVTHNVPIYHAIMRLDLA.
When using antibody 2G2 in immunolocalization studies the presence of nuclear actin could be demonstrated e.g. in cultured cells after fixation with 4% paraformaldehyde. Immunostaining with cytoplasmic actin was only positive after fixation with methanol (Gonsior et al., 1999).
Reactivity on cultured cell lines: PtK2, SR-NRK, NRK-49F, L6 cells, rat fibroblasts, NIH-3T3, mouse myoblast and myotube cells.
Gonsior SM, Platz S, Buchmeier S, Scheer U, Jockusch BM, Hinssen H: Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody. J Cell Sci 112, 797-809 (1999)
Reference
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ICC-IF
rat
dot blot
mouse
bovine
ICC-IF
pleurodeles
IEM