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anti-Actin mouse monoclonal, 2G2, supernatant

Specific for skeletal and non-muscle actin isoforms (Mr 43,000). The antibody also detects nuclear actin isoforms. Epitope location: NVPAMYVAVLDSGVTHNVPIYHAIMRLDLA.
Cat. No.: 651132
Quantity:  5 mL
No. of experiments:  ≤ 100 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$293.00
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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgM
Clone 2G2
Immunogen profilin actin complex from calf thymus
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 100 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity bovine, chicken, human, plant, rabbit

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent (IF only after methanol fixation)
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (protease treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Specific for skeletal and non-muscle actin isoforms (Mr 43,000). Epitope location: NVPAMYVAVLDSGVTHNVPIYHAIMRLDLA.

When using antibody 2G2 in immunolocalization studies the presence of nuclear actin could be demonstrated e.g. in cultured cells after fixation with 4% paraformaldehyde. Immunostaining with cytoplasmic actin was only positive after fixation with methanol (Gonsior et al., 1999).

Reactivity on cultured cell lines: PtK2, SR-NRK, NRK-49F, L6 cells, rat fibroblasts, NIH-3T3, mouse myoblast and myotube cells.

Gonsior SM, Platz S, Buchmeier S, Scheer U, Jockusch BM, Hinssen H: Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody. J Cell Sci 112, 797-809 (1999)

Reference

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