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anti-Actin mouse monoclonal, 2G2, supernatant Zoom

anti-Actin mouse monoclonal, 2G2, supernatant

Specific for skeletal and non-muscle actin isoforms (Mr 43,000). The antibody also detects nuclear actin isoforms. Epitope location: NVPAMYVAVLDSGVTHNVPIYHAIMRLDLA.
Cat. No.: 651132
Quantity:  5 mL
No. of experiments:  ≤ 100 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$293.00
Excl. VAT, excl. Shipping Cost

Filter

Publication
Species
Application
Hensen, F. et al. Mitochondrial RNA granules are critically dependent on mtDNA replication factors Twinkle and mtSSB. Nucleic.Acids.Res. , (2019)
human
ICC-IF
Domke, L and Franke, W. The cell-cell junctions of mammalian testes.., Cell Tissue Res, 375, 451-482, (2019)
pig
ICC-IF
Cruz, J. R. & Moreno Diaz de la Espina, S. Subnuclear compartmentalization and function of actin and nuclear Myosin I in plants. Chromosoma 118, 193-207 (2009).
A. cepa
WB
ICC-IF
Jockusch, B. M., Schoenenberger, C. A., Stetefeld, J. & Aebi, U. Tracking down the different forms of nuclear actin. Trends Cell Biol. 16, 391-396 (2006).
human
rat
ICC-IF
Schoenenberger, C. A. et al. Conformation-specific antibodies reveal distinct actin structures in the nucleus and the cytoplasm. J. Struct. Biol. 152, 157-168 (2005).
human
rat
ICC-IF
dot blot
Bettinger, B. T., Gilbert, D. M. & Amberg, D. C. Actin up in the nucleus. Nat. Rev. Mol. Cell Biol. 5, 410-415 (2004).
human
mouse
bovine
WB
ICC-IF
Peitsch, W. K. Cell biological and biochemical characterization of drebrin complexes in mesangial cells and podocytes of renal glomeruli. J. Am. Soc. Nephrol. 14, 1452-1463 (2003).
mouse
ICC-IF
Zhang, S. et al. Nuclear DNA helicase II/RNA helicase a binds to filamentous actin. J. Biol. Chem. 277, 843-853 (2002).
human
WB
IEM
Hofmann, W. et al. Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs: An unexpected role for actin. J. Cell Biol. 152, 895-910 (2001).
xenopus
pleurodeles
WB
IEM
Gonsior, S. M. et al. Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody. J. Cell Sci. 112 ( Pt 6, 797-809 (1999).
human
WB

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgM
Clone 2G2
Immunogen profilin actin complex from calf thymus
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 100 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity bovine, chicken, human, plant, rabbit

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent (IF only after methanol fixation)
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (protease treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Specific for skeletal and non-muscle actin isoforms (Mr 43,000). Epitope location: NVPAMYVAVLDSGVTHNVPIYHAIMRLDLA.

When using antibody 2G2 in immunolocalization studies the presence of nuclear actin could be demonstrated e.g. in cultured cells after fixation with 4% paraformaldehyde. Immunostaining with cytoplasmic actin was only positive after fixation with methanol (Gonsior et al., 1999).

Reactivity on cultured cell lines: PtK2, SR-NRK, NRK-49F, L6 cells, rat fibroblasts, NIH-3T3, mouse myoblast and myotube cells.

Gonsior SM, Platz S, Buchmeier S, Scheer U, Jockusch BM, Hinssen H: Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody. J Cell Sci 112, 797-809 (1999)

Reference

Filter

Publication
Species
Application
Hensen, F. et al. Mitochondrial RNA granules are critically dependent on mtDNA replication factors Twinkle and mtSSB. Nucleic.Acids.Res. , (2019)
human
ICC-IF
Domke, L and Franke, W. The cell-cell junctions of mammalian testes.., Cell Tissue Res, 375, 451-482, (2019)
pig
ICC-IF
Cruz, J. R. & Moreno Diaz de la Espina, S. Subnuclear compartmentalization and function of actin and nuclear Myosin I in plants. Chromosoma 118, 193-207 (2009).
A. cepa
WB
ICC-IF
Jockusch, B. M., Schoenenberger, C. A., Stetefeld, J. & Aebi, U. Tracking down the different forms of nuclear actin. Trends Cell Biol. 16, 391-396 (2006).
human
rat
ICC-IF
Schoenenberger, C. A. et al. Conformation-specific antibodies reveal distinct actin structures in the nucleus and the cytoplasm. J. Struct. Biol. 152, 157-168 (2005).
human
rat
ICC-IF
dot blot
Bettinger, B. T., Gilbert, D. M. & Amberg, D. C. Actin up in the nucleus. Nat. Rev. Mol. Cell Biol. 5, 410-415 (2004).
human
mouse
bovine
WB
ICC-IF
Peitsch, W. K. Cell biological and biochemical characterization of drebrin complexes in mesangial cells and podocytes of renal glomeruli. J. Am. Soc. Nephrol. 14, 1452-1463 (2003).
mouse
ICC-IF
Zhang, S. et al. Nuclear DNA helicase II/RNA helicase a binds to filamentous actin. J. Biol. Chem. 277, 843-853 (2002).
human
WB
IEM
Hofmann, W. et al. Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs: An unexpected role for actin. J. Cell Biol. 152, 895-910 (2001).
xenopus
pleurodeles
WB
IEM
Gonsior, S. M. et al. Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody. J. Cell Sci. 112 ( Pt 6, 797-809 (1999).
human
WB

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