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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgM |
| Clone | 647 |
| Immunogen | antigen isolated from chicken skeletal muscle cells |
| Purification | hybridoma cell culture supernatant |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 200 (IHC, 50 µl/slide) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | chicken, human, mouse, rat |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:5 - 1:10 |
| Immunohistochemistry (IHC) - paraffin | 1:5 - 1:10 (microwave treatment recommended) |
| Western Blot (WB) | assay dependent |
Background
Details
Mab 647 is useful for studying the intracellular distribution and structure of actin in the cytoskeletal system. In immunoblotting one single band is reactive corresponding to actin.
Positive control: Muscle or myofibrils.
Reference
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