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anti-Actin mouse monoclonal, 647, supernatant

Reacts specifically with actin in Immunoblotting and ELISA. It is intended for use in immunostaining of cell suspensions and acetone-fixed frozen tissue sections.
Cat. No.: 10601
Quantity:  1 mL
No. of experiments:  ≤ 200 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$306.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgM
Clone 647
Immunogen antigen isolated from chicken skeletal muscle cells
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 200 (IHC, 50 µl/slide)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity chicken, human, mouse, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:5 - 1:10
Immunohistochemistry (IHC) - paraffin 1:5 - 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Mab 647 is useful for studying the intracellular distribution and structure of actin in the cytoskeletal system. In immunoblotting one single band is reactive corresponding to actin.

Positive control: Muscle or myofibrils.

Reference

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