Epitope mapped
Frequently used
Diagnostic marker

anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ASM-1, lyophilized, purified

Liquid

Liquid

Lyophilized

Prediluted

Cat. No. 61001

Available, delivery time 1-7 days

€226.00*

Key Features
  • Purified, lyophilized
  • Mouse monoclonal
  • Suitable for ICC/IF, IHC and WB
  • Reacts with bovine, chicken, equine, human, mouse, rat
  • Isotype: IgG2a
Product description
Quantity 50 µg
Antibody Type Monoclonal
Host Mouse
Isotype IgG2a
Conjugate Unconjugated
Application ICC/IF, IHC, WB
Purification Affinity chromatography
Reactivity Bovine, Chicken, Horse, Human, Mouse, Rat
Storage before reconstitution 2-8°C until indicated expiry date
Storage after reconstitution Up to 3 months at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use Research use only
Clone 1A4/ASM-1
Immunogen Synthetic N-terminus decapeptide of alpha-smooth-muscle isoform of actin
Concentration 50 µg/ml after reconstitution with 1 ml dist. water
Formulation Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
UniprotID P62739 (Bovine),P08023 (Chicken),P62736 (Human),P62737 (Mouse),P62738 (Rat)
Synonym Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein [Cleaved into: Actin, aortic smooth muscle, intermediate form], ACTA2, ACTSA, ACTVS, GIG46
Applications
Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) Assay dependent
Immunohistochemistry (IHC) - frozen 1:100-1:500
Immunohistochemistry (IHC) - paraffin 1:100-1:500 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) 1:500
Background

1A4/ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation.
Polypeptide reacting: specific for alpha-smooth-muscle isoform of actin (43 kDa).
Tumors specificallly detected: leiomyosarcoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast.
Tested cultured cell lines: Stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts

References/Publications (21)
Publication
Species
Application
Downloads
File
Category
Size
Filetype
Category Protocol
Size 919.99 KB
Filetype pdf
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