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Product description
| Host | rabbit |
|---|---|
| Antibody Type | polyclonal |
| Immunogen | Synthetic alpha-MSH (Sigma Chem. Co., USA) conjugated to BSA |
| Purification | undiluted antiserum |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 100 µl dist. water |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 800 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | pig, rat |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:200 - 1:400 |
| Immunohistochemistry (IHC) - paraffin | 1:200 - 1:400 (microwave treatment recommended) |
Background
Details
Specific for alpha-melanocyte stimulating hormone. Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining.
In man, a-MSH is found in corticotrophs of the anterior pituitary and may also occur in brain neurons.
Positive control: Bouin-fixed paraffin sections of pig pituitary
Reference
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