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WB anti-alpha smooth muscle actin (Cat. No. 61001) Zoom

WB anti-alpha smooth muscle actin (Cat. No. 61001)

anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ ASM-1, lyophilized, purified

1A4/ ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. Stains leiomyosarkoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast.
application_confirmed highly_published
Cat. No.: 61001
Quantity:  50 µg
No. of experiments:  ≤ 2000 (IHC, 50 µl/slide); ≤ 100 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$274.00
Excl. VAT, excl. Shipping Cost

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Specifications

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65001

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690001

anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ ASM-1, liquid, purified

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anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ ASM-1, liquid, purified, small

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a
Clone 1A4/ ASM-1
Immunogen synthetic N-terminus decapeptide of α-smooth-muscle isoform of actin
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 2000 (IHC, 50 µl/slide); ≤ 100 (WB, 5 ml reaction volume)
Intended use Research use only
Tested species reactivity bovine, chicken, equine, human, mouse, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen dilute at least 1:100
Immunohistochemistry (IHC) - paraffin dilute at least 1:100 (protease treatment and/or microwave treatment recommended)
Western Blot (WB) 1:500

Background

Details

1A4/ ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation.
Polypeptide reacting: specific for α-smooth-muscle isoform of actin (Mr 43,000).
Tumors specificallly detected: leiomyosarcoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast.
Tested cultured cell lines: Stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts

Reference

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