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anti-alpha-Smooth Muscle Actin mouse monoclonal, ASM-1, prediluted, purified

ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. Stains leiomyosarkoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast.
Cat. No.: 65001
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€129.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a
Clone ASM-1
Immunogen synthetic N- terminus decapeptide of -smooth-muscle isoform of actin
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, chicken, equine, human, mouse, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (protease treatment and/or microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation.
Polypeptide reacting: specific for alpha-smooth-muscle isoform of actin (Mr 43,000).
Tumors specificallly detected: leiomyosarcoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast.
Reactive cultured cell lines: Stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts

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