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anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ ASM-1, prediluted, purified

1A4/ ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. Stains leiomyosarkoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast.
Cat. No.: 65001
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€129.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a
Clone 1A4/ ASM-1
Immunogen synthetic N- terminus decapeptide of -smooth-muscle isoform of actin
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, chicken, equine, human, mouse, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (protease treatment and/or microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

1A4/ ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation.
Polypeptide reacting: specific for alpha-smooth-muscle isoform of actin (Mr 43,000).
Tumors specificallly detected: leiomyosarcoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast.
Reactive cultured cell lines: Stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts

Reference

Reference

PublicationSpeciesApplication
Zac-Varghese, S. et al. The Peutz-Jeghers kinase LKB1 suppresses polyp growth from intestinal cells of a proglucagon-expressing lineage in mice. Dis. Model. Mech. 7, 1275–86 (2014). mouse IHC (frozen)
Takaba, K. et al. Spontaneous Adenosquamous Carcinoma with Rapid Growth and EMT-like Changes in the Mammary Gland of a Young Adult Female BALB/c Mouse. J. Toxicol. Pathol. 25, 265–271 (2012). human IHC (paraffin)
Van Es, L. et al. GMP-17-positive T-lymphocytes in renal tubules predict progression in early stages of IgA nephropathy. Kidney Int. 73, 1426–1433 (2008). human IHC (paraffin)
Yasui, F., Miyazu, M., Yoshida, A., Naruse, K. & Takai, A. Examination of signalling pathways involved in muscarinic responses in bovine ciliary muscle using YM-254890, an inhibitor of the G q/11 protein. Br. J. Pharmacol. 154140, 890–900 (2008). bovine ICC-IF
Ehler, E., Fowler, V. M. & Perriard, J.-C. Myofibrillogenesis in the developing chicken heart: Role of actin isoforms and of the pointed end actin capping protein tropomodulin during thin filament assembly. Dev. Dyn. 229, 745–755 (2004). chicken WB, whole mount
Rudy, D. E., Yatskievych, T. A., Antin, P. B. & Gregorio, C. C. Assembly of thick, thin, and titin filaments in chick precardiac explants. Dev. Dyn. 221, 61–71 (2001). chicken ICC-IF
Demirkesen, C., Hoede, N. & Moll, R. Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins. J. Cutan. Pathol. 22, 518–35 (1995). human IHC (paraffin)
Schurch, W., Skalli, O., Seemayer, T. A. & Gabbiani, G. Intermediate Filament Proteins and Actin Isoforms as Markers for Soft Tissue Tumor Differentiation and Origin I. Smooth Muscle Tumors. Am. J. Pathol. 128, 91–103 (1987). human IHC (frozen)
Franke, W. W. & Moll, R. Cytoskeletal components of lymphoid organs. I. Synthesis of cytokeratins 8 and 18 and desmin in subpopulations of extrafollicular reticulum cells of human lymph nodes, tonsils, and spleen. Differentiation. 36, 145–63 (1987). human IHC (frozen)
Skalli, O. et al. A Monoclonal Antibody against a-Smooth Muscle Actin: A New Probe for Smooth Muscle Differentiation. J. Cell Biol. 103, 2787–2796 (1986). human, rat, bovine, chicken WB, IHC (frozen)

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