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anti-Androgen Receptor mouse monoclonal, F39.4, purified

The antibody is directed against the N-terminal domain of the human androgen receptor. AR is expressed e.g. in the testis, (Sertoli and Leydig cells), fibroblasts, breast epithelium, in hepatocytes and the prostate.
Cat. No.: 16041
Quantity:  1 mL
No. of experiments:  ≤ 200 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone F39.4
Immunogen synthetic peptide (comprising amino acids 301-320 of human androgen receptor)
Purification affinity chromatography
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 200 (IHC, 50 µl/slide)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human


Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:5 - 1:10
Immunohistochemistry (IHC) - paraffin 1:5 - 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent



The antibody is reactive with a 110 kD protein in a lysate from the LNCP cell line. The androgen receptor (AR) has been localized e.g. in the testis, (Sertoli and Leydig cells), prostate, foreskin epidermis, fibroblasts, breast epithelium, sweat and apocrine glands and hepatocytes.
Prostate cancers are very heterogeneous with regard to AR expression, the loss of androgen responsiveness is not paralleled by the loss of AR. Studies on AR distribution might be useful to study the role of AR in related disease processes.
Antigen localization is nuclear.

Positive control: Testis.


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