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human beard hair (courtesy of L. Langbein) Zoom

human beard hair (courtesy of L. Langbein)

anti-basic Hair Keratin K83 guinea pig polyclonal, serum

Guinea pig polyclonal anti-basic Hair Keratin K83 antibody.
Cat. No.: GP-HHB3
Quantity:  100 µL
No. of experiments:  ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$325.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen Synthetic peptide of human basic hair (trichocytic) keratin K83 (formerly also designated hHb3; CGFNSIGCGFRPGNF), coupled to KLH
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity human, mouse

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:200 (for enhancement of cortex staining preincubate fixed sections with 0.1 Triton X-100 (in PBS) for 1 – 5 min prior to first antibody incubation step)
Western Blot (WB) 1:2,000

Background

Details

The antiserum stains specifically human hair keratin K83 expressed starting from the middle cortex.

Reference

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