human scalp hair (courtesy of L. Langbein)

anti-basic Hair Keratin K86 guinea pig polyclonal, serum

The antibody stains specifically human hair keratin hHb6 expressed starting from the middle hair cortex. It can be defect in monilethrix.

Cat. No.: GP-HHB6
Quantity: 100 µL
No. of experiments: ≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$325.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Langbein, L., Yoshida, H., Praetzel-Wunder, S., Parry, D. A. & Schweizer, J. The Keratins of the Human Beard Hair Medulla: The Riddle in the Middle. J. Invest. Dermatol. 130, 55â73 (2010).

human

IHC (frozen)

Langbein, L., Spring, H., Rogers, M. A., Praetzel, S. & Schweizer, J. Hair keratins and hair follicle-specific epithelial keratins. Methods Cell Biol 78, 413â451 (2004).

human

Langbein, L., Rogers, M. A., Winter, H., Praetzel, S. & Schweizer, J. The catalog of human hair keratins: II. Expression of the six type II members in the hair follicle and the combined catalog of human type I and II keratins. J. Biol. Chem. 276, 35123â35

human

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
GP-HHB6S	anti-basic Hair Keratin K86 guinea pig polyclonal	serum, sample (ready-to-use)	600 µL (ready-to-use)	$65.00 Excl. VAT, excl. Shipping Cost
70022	anti-hair Keratin sample set		600 µl each antibody	$230.00

Product description

Host	guinea pig
Antibody Type	polyclonal
Immunogen	synthetic peptide of human basic hair (trichocytic) keratin K86 (formerly also designated hHb6; APV VST RVS SVP SNS NVV VGT TNA C), coupled to KLH
Purification	stabilized antiserum
Conjugate	unconjugated
Formulation	contains 0.09% sodium azide and 0.5% BSA
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments	≤ 400 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)
Note	centrifuge prior to opening
Intended use	Research use only
Tested species reactivity	human, mouse

Applications

Tested applications	Tested dilutions
Immunohistochemistry (IHC) - frozen	1:200 (for enhancement of cortex staining preincubate fixed sec-tions with 0.1 Triton X-100 (in PBS) for 1 – 5 min prior to first antibody incubation step)
Western Blot (WB)	1:2,000

Background

Details

The antiserum stains specifically human hair keratin K86 expressed starting from the middle hair cortex. It can be defect in Monilethrix.

Reference

Publication

Species

Application

Langbein, L., Yoshida, H., Praetzel-Wunder, S., Parry, D. A. & Schweizer, J. The Keratins of the Human Beard Hair Medulla: The Riddle in the Middle. J. Invest. Dermatol. 130, 55â73 (2010).

human

IHC (frozen)

Langbein, L., Spring, H., Rogers, M. A., Praetzel, S. & Schweizer, J. Hair keratins and hair follicle-specific epithelial keratins. Methods Cell Biol 78, 413â451 (2004).

human

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anti-basic Hair Keratin K86 guinea pig polyclonal, serum

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