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anti-Bromodeoxyuridine mouse monoclonal, Bu5.1, lyophilized, purified

For identification of S-phase cells and detection of bromodeoxyuridine incorporation in tissue and cell culture.
Cat. No.: 61015
Quantity:  100 µg

Delivery Time: usually 1-7 working days

$251.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

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61016

anti-Bromodeoxyuridine mouse monoclonal, Bu5.1

FITC Conjugate 1.5 mL
$311.00
Excl. 19% Tax, excl. Shipping Cost

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a
Clone Bu5.1
Immunogen Bromodeoxyuridine
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity tissue and cell culture after incorporation of BrdU

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:10
Immunohistochemistry (IHC) - frozen 1:10 (proteolytic treatment recommended)
Immunohistochemistry (IHC) - paraffin 1:10 (proteolytic treatment and/or microwave treatment recommended)

Background

Details

The antibody is specific for bromodesoxyuridine and does not cross-react with thymidine.
It is a valuable tool for detection of S-phase (DNA-synthesizing cells) in cell ad tissue culture. 3H-thymi­dine labelling techniques can be avoided.

Reference

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