Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG2a kappa |
Clone | 102.43 |
Immunogen | Butyrophilin purified from bovine milk fat globule membrane |
Purification | hybridoma cell culture supernatant |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 100 (IHC, 50 µl/slide) |
Intended use | Research use only |
Tested species reactivity | bovine, human |
Applications
Tested applications | Tested dilutions |
---|---|
Immunohistochemistry (IHC) - frozen | ready-to-use |
Western Blot (WB) | assay dependent |
Background
Details
The mab reacts specifically with butyrophilin, a unique 67 kD polypeptide present in lactating mammary gland epithelium and in the milk fat globule membrane (MFGM).
No cross-reaction with other MFGM proteins. The epitope has been localized on the N-terminal extracellular part of the protein, close to the transmembrane region.
Butyrophilins have recently been described as a new family of immunoregulators, similar to the co-stimulatory and co-inhibitory family of B7 molecules (Arnett & Viney, 2014).
Polypeptide reacting:Butyrophilin, 67 kD polypeptide; present in the apical membrane of lactating mammary epithelium and in MFGM.
Reactivity on cultured cell lines: Transfected cell line BMGE with butyrophilin cDNA.
Positive control: Milk fat globule membrane (MFGM).
Arnett HA & Viney JL: Immune modulation by butyrophilins. Nature Review Immunology 14, 559-569 (2014)