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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | 2B6 |
| Immunogen | GST fusion protein of part of the extracellular domain of cadherin 6 (K-Cadherin) |
| Purification | hybridoma cell culture supernatant |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles |
| Tested species reactivity | human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | ready-to-use (paraformaldehyde fixed; with 0.2% Triton X-100 in PBS) |
Background
Details
The cadherin gene family encodes calcium-dependent adhesion molecules that promote homophilic interactions among cells. All cadherins are single-pass transmembrane proteins with a variable number of extracellular domains (EC; 110 amino acids long). Classical cadherins consist of five EC domains and a conserved cytoplasmic domain and are divided in type I and type II subgroups. Type I comprises cadherin E, N, P, and R. Type II include cadherin-5, -6, -8, -11, and -12.
During embryogenesis differential expression of cadherins can drive morphogenesis by stimulating cell aggregation, defining boundaries between groups of cells and promoting cell migration. Cadherin-6 seems to be required for the early aggregation of induced mesenchymal cells and their subsequent conversion to epithelium.
Positive control: Embryonic kidney (e.g. establishment of developmental pattern of nephrons)