Product description
Host | mouse |
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Antibody Type | monoclonal |
Isotype | IgG1 |
Clone | 2B6 |
Immunogen | GST fusion protein of part of the extracellular domain of cadherin 6 (K-Cadherin) |
Purification | hybridoma cell culture supernatant |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide |
Storage | short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles |
Tested species reactivity | human |
Note | centrifuge prior to opening |
Intended use | Research use only |
Applications
Tested applications | Tested dilutions |
---|---|
Immunohistochemistry (IHC) - frozen | ready-to-use (paraformaldehyde fixed; with 0.2% Triton X-100 in PBS) |
Background
Details
The cadherin gene family encodes calcium-dependent adhesion molecules that promote homophilic interactions among cells. All cadherins are single-pass transmembrane proteins with a variable number of extracellular domains (EC; 110 amino acids long). Classical cadherins consist of five EC domains and a conserved cytoplasmic domain and are divided in type I and type II subgroups. Type I comprises cadherin E, N, P, and R. Type II include cadherin-5, -6, -8, -11, and -12.
During embryogenesis differential expression of cadherins can drive morphogenesis by stimulating cell aggregation, defining boundaries between groups of cells and promoting cell migration. Cadherin-6 seems to be required for the early aggregation of induced mesenchymal cells and their subsequent conversion to epithelium.
Positive control: Embryonic kidney (e.g. establishment of developmental pattern of nephrons)