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anti-Calcitonin Gene Related Peptide rabbit polyclonal, serum

CGRP is present in the C-cells of the thyroid and in nerves in the brain and periphery. Medullary carcinomas of the thyroid show large amounts of CGRP. The antiserum does not cross-react with amylin.
Cat. No.: 16012
Quantity:  50 µL

Delivery Time: usually 1-7 working days

€411.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host rabbit
Antibody Type polyclonal
Immunogen Calcitonin gene-related peptide, rat (#6006, Peninsula)
Purification stabilized antiserum
Conjugate unconjugated
Formulation lyophilized; reconstitute in 50 - 100 μl dist. water (final solution contains 0.09 % sodium azide, 1% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, mouse, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:800 - 1:2,000
Immunohistochemistry (IHC) - paraffin 1:800 - 1:2,000 (microwave treatment recommended)

Background

Details

The CGRP-sequence was predicted from the corresponding mRNA. CGRP is present in the C-cells of the thyroid and in nerves, in both brain and periphery, particularly within the sensory system. CGRP is a potent vasodilator, probably involved in neurogenic inflammation. Medullary carcinomas of the thyroid contain large amounts of CGRP. Absorption with 10 – 100 µg CGRP per ml diluted antiserum abolishes the staining while calcitonin does not.  Does not cross-react with amylin (IAPP).
Positive control: Frozen sections of rat colon.

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