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anti-Calcitonin Gene Related Peptide (rat) guinea pig polyclonal, serum

CGRP is present in the C-cells of the thyroid and in nerves in the brain and periphery. Medullary carcinomas of the thyroid show large amounts of CGRP. The antiserum does not cross-react with amylin.
Cat. No.: 16013
Quantity:  50 µL

Delivery Time: usually 1-7 working days

€411.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen Calcitonin gene-related peptide (synthetic, rat, (#6006, Peninsula), conjugated to BSA
Purification undiluted antiserum
Conjugate unconjugated
Formulation lyophilized; reconstitute in 100 µl dist. water
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity human, mouse, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:800 - 1:1,200
Immunohistochemistry (IHC) - paraffin 1:800 - 1:1,200 (microwave treatment recommended)

Background

Details

The CGRP-sequence was predicted from the corresponding mRNA. CGRP is present in the C-cells of the thyroid and in nerves, in both brain and periphery, particularly within the sensory system. CGRP is a potent vasodilator, probably involved in neurogenic inflammation. Medullary carcinomas of the thyroid contain large amounts of CGRP. Absorption with 10 – 100 µg CGRP per ml diluted antiserum abolishes the staining while calcitonin does not. Does not cross-react with amylin (IAPP).
Positive control: Frozen sections of rat colon.

Reference

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