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anti-Calicin (N-terminus) guinea pig polyclonal, serum

Useful to study spermiogenetic differentiation. Calicin is absent from pathologic forms like round-headed or other post-acrosomal sheath defect sperm malformations.
Cat. No.: GP-SH3
Quantity:  100 µL
No. of experiments:  ≤ 200 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$319.00
Excl. VAT, excl. Shipping Cost

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Specifications

Product description

Host guinea pig
Antibody Type polyclonal
Immunogen synthetic N-terminal domain (aa 1-24) of bovine calicin, coupled to KLH
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 200 (IHC, 50 µl/slide); ≤ 40 (WB, 5 ml reaction volume)
Note centrifuge prior to opening
Intended use Research use only
Tested species reactivity bovine, human, mouse, rabbit, rat

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:100
Western Blot (WB) 1:2,000

Background

Details

Reactive polypeptide (specificity): Sperm head calyx protein calicin (mw 66,889; IEP 8.1; 588 amino acids), testis specific cytoskeletal protein
Homologue to kelch protein of the ring canal structure of Drosophila ovaries. Useful to study spermiogenetic differentiation. Calicin is absent from pathologic forms like round-headed or other post-acrosomal sheath defect sperm malformations.

Reference

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