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Calprotectin positive cells in tumor stroma. Zoom

Calprotectin positive cells in tumor stroma.

anti-Calprotectin S100A8/A9 Complex mouse monoclonal, 69-6-1, lyophilized, purified

Mouse monoclonal anti-Calprotectin antibody. Recognizes the S100A8/S100A9 heterocomplex.
Cat. No.: 610191
Quantity:  100 µg
No. of experiments:  ≤ 6000 (IHC, 50 µl/slide); ≤ 200 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone 69-6-1
Immunogen recombinant protein
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 6000 (IHC, 50 µl/slide); ≤ 200 (WB, 5 ml reaction volume)
Intended use Research use only
Tested species reactivity human


Tested applications Tested dilutions
ELISA 1:50
Immunohistochemistry (IHC) - paraffin 1:100 – 1:300 (microwave treatment recommended)
Western Blot (WB) 1:1,000



Calprotectin is a functional heterocomplex of small EF hand calcium binding proteins S100A8 and S100A9. The heterocomplex is expressed in various cells and tissues and induced upon inflammation. The complex is released by neutrophilic granulocytes in inflammatory disorders such as inflammatory bowel disease and rheumatoid arthritis. Released Calprotectin serves as a leukocyte chemoattractant and modulates inflammation via damage receptors. Via it's binding to the receptor of advanced glycation end-products (RAGE), calprotectin is implicated in chronic inflammation and was also linked to cancer formation.

Tissue expression: strong expression in myeloid cells and psoriatic keratinocytes.


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