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anti-Calprotectin S100A9 & S100A8/A9 Complex mouse monoclonal, 23-3-4, lyophilized, purified

Mouse monoclonal antibody specific to S100A9 and the S100A8/A9 heterocomplex
Cat. No.: 610188
Quantity:  100 µg

Delivery Time: usually 1-7 working days

Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone 23-3-4
Immunogen recombinant protein
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 mL dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human
Intended use Research use only


Tested applications Tested dilutions
ELISA 1:50
Immunohistochemistry (IHC) - paraffin 1:300 - 1:1,000 (microwave treatment recommended)
Western Blot (WB) 1:1,000



Calprotectin is a functional heterocomplex of small EF hand calcium‑binding proteins S100A8 and S100A9. The heterocomplex is expressed in various cell types and tissues and induced upon inflammation. The complex is released by neutrophilic granulocytes in inflammatory disorders such as inflammatory bowel disease and rheumatoid arthritis. Released Calprotectin serves as a leukocyte chemoattractant and modulates inflammation via damage receptors. Via it's binding to the receptor of advanced glycation end-products (RAGE), calprotectin is implicated in chronic inflammation and cancer formation.

Tissue expression: strong expression in myeloid cells and psoriatic keratinocytes.

The antibody recognizes both the S100A8/A9 heterocomplex as well as S100A9 alone but not S100A8.

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