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Cardiac actin staining on human heart muscle (courtesy of The Human Protein Atlas, www.proteinatlas.org, Thul PJ et al, 2017. A subcellular map of the human proteome. Science) Zoom

Cardiac actin staining on human heart muscle (courtesy of The Human Protein Atlas, www.proteinatlas.org, Thul PJ et al, 2017. A subcellular map of the human proteome. Science)

anti-Cardiac Actin mouse monoclonal, AC1-20.4.2, lyophilized, purified

Marker for the discrimination of cardiac (fetal) alpha-actin from other actin isoforms present in skeletal and smooth muscle. Fetal actin can be localized in regenerating muscle after injury (satellite cells).
Cat. No.: 61075
Quantity:  50 µg
No. of experiments:  ≤ 200 (IHC, 50 µl/slide); ≤ 200 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$251.00
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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone AC1-20.4.2
Immunogen synthetic NH2 terminus decapeptide of cardiac isoform of actin
Purification affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 200 (IHC, 50 µl/slide); ≤ 200 (WB, 5 ml reaction volume)
Intended use Research use only
Tested species reactivity bovine, chicken, human, rabbit

Applications

Tested applications Tested dilutions
ELISA 1:500 (include 1 M NaCl in all washing buffers to enhance specificity)
Immunohistochemistry (IHC) - frozen 1:10 (include 0.5 M NaCl in all washing buffers to enhance specificity)
Immunohistochemistry (IHC) - paraffin 1:10 (microwave treatment recommended)
Western Blot (WB) 1:1,000 (include 1 M NaCl in all washing buffers to enhance specificity)

Background

Details

Ac1 represents an excellent marker for cardiac tissue; it discriminates fetal (cardiac) a-actin from all other actin isoforms. Fetal actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord. Mab Ac1-20.4.2 shows no cross reaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied.
Polypeptide reacting: Specific for fetal (cardiac) isoform of actin.

Reference

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