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anti-Cardiac Actin mouse monoclonal, AC1-20.4.2, supernatant

Marker for the discrimination of cardiac (fetal) alpha-actin from other actin isoforms present in skeletal and smooth muscle. Fetal actin can be localized in regenerating muscle after injury (satellite cells).
Cat. No.: 65175
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€133.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone AC1-20.4.2
Immunogen synthetic NH2 terminus decapeptide of cardiac isoform of actin
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, chicken, human, rabbit

Applications

Tested applications Tested dilutions
ELISA 1:50
Immunohistochemistry (IHC) - frozen ready-to-use (to enhance specificity the addtions of 0.5 M NaCl in all washing buffers is recommended)
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) 1:20

Background

Details

Ac1 represents an excellent marker for cardiac tissue; it discriminates fetal (cardiac) alpha-actin from all other actin isoforms. Fetal actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord.
Mab Ac1-20.4.2 shows no cross reaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied.

Polypeptide reacting: Specific for cardiac isoform of actin.

Reference

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