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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG2b |
| Clone | MO-1.1 |
| Immunogen | recombinant C-terminal 221 aa fragment of human wild-type cdk7/CAK (cyclin activating kinase) |
| Purification | protein A affinity chromatography |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 1 mL dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 400 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | human, mouse |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Flow Cytometry (FACS) | assay dependent |
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:10 - 1:20 |
| Immunoprecipitation (IP) | assay dependent |
| Western Blot (WB) | assay dependent |
Background
Details
Mo-1.1 recognizes the key cell cycle regulator cdk7, which is essential to activate the cdk2 kinase in G1 phase and the cdc2 kinase in G2/M phase. The antibody is useful for cell cycle analysis, cell proliferation studies and for immunohistochemistry.
Reference
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