anti-Channelrhodopsin 2 antibody in western blot

anti-Channelrhodopsin 2 mouse monoclonal, 15E2, supernatant

The antibody specifically detects an epitope at the C terminus of Channelrhodopsin 2 (ChR2) (aa 290–309).

Cat. No.: 651180
Quantity: 5 mL
No. of experiments: ≤ 200 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$251.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Weiglein, A. et al. One-trial learning in larval Drosophila. Learn.Mem. 26, 109-120 (2019)

drosophila

IHC

Wang, Y. et al. Optogenetic Control of Heart Rhythm by Selective Stimulation of Cardiomyocytes Derived from Pnmt + Cells in Murine Heart. Sci. Rep. 7, 1â10 (2017).

mouse

IHC-IF

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Specifications

Datasheet

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
610180	anti-Channelrhodopsin 2 mouse monoclonal, 15E2	lyophilized, purified	50 µg	$385.00 Excl. VAT, excl. Shipping Cost

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG2a
Clone	15E2
Immunogen	purified recombinant ChR2-315
Purification	hybridoma cell culture supernatant
Conjugate	unconjugated
Formulation	contains 0.09% sodium azide
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments	≤ 200 (WB, 5 ml reaction volume)
Intended use	Research use only
Tested species reactivity	Chlamydomonas

Applications

Tested applications	Tested dilutions
ELISA	1:10 - 1:500
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	assay dependent
Immunohistochemistry (IHC) - frozen	assay dependent
Immunohistochemistry (IHC) - paraffin	no data available
Western Blot (WB)	1:100 - 1:200

Background

Details

Channelrhodopsin 2 (ChR2) is a cation-permeable channel protein that enables cell depolarisation in response to blue light. The antibody specifically detects the C terminus of ChR2. The epitope is located intracellularly between amino acids 290 and 309 of Chr2.

Reference