anti-Channelrhodopsin 2 antibody in western blot

anti-Channelrhodopsin 2 mouse monoclonal, 15E2, supernatant

The antibody specifically detects an epitope at the C terminus of Channelrhodopsin 2 (ChR2) (aa 290–309).

Cat. No.: 651180
Quantity: 5 mL
No. of experiments: ≤ 200 (WB, 5 ml reaction volume)

Delivery Time: usually 1-7 working days

$251.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Wang, Y. et al. Optogenetic Control of Heart Rhythm by Selective Stimulation of Cardiomyocytes Derived from Pnmt + Cells in Murine Heart. Sci. Rep. 7, 1â10 (2017).

mouse

IHC-IF

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Specifications

Datasheet

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Cat. No.	Product Name	Format	Quantity	Price
610180	anti-Channelrhodopsin 2 mouse monoclonal, 15E2	lyophilized, purified	50 µg	$385.00 Excl. VAT, excl. Shipping Cost

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG2a
Clone	15E2
Immunogen	purified recombinant ChR2-315
Purification	hybridoma cell culture supernatant
Conjugate	unconjugated
Formulation	contains 0.09% sodium azide
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments	≤ 200 (WB, 5 ml reaction volume)
Intended use	Research use only
Tested species reactivity	Chlamydomonas

Applications

Tested applications	Tested dilutions
ELISA	1:10 - 1:500
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	assay dependent
Immunohistochemistry (IHC) - frozen	assay dependent
Immunohistochemistry (IHC) - paraffin	no data available
Western Blot (WB)	1:100 - 1:200

Background

Details

Channelrhodopsin 2 (ChR2) is a cation-permeable channel protein that enables cell depolarisation in response to blue light. The antibody specifically detects the C terminus of ChR2. The epitope is located intracellularly between amino acids 290 and 309 of Chr2.

Reference

Publication

Species

Application

Wang, Y. et al. Optogenetic Control of Heart Rhythm by Selective Stimulation of Cardiomyocytes Derived from Pnmt + Cells in Murine Heart. Sci. Rep. 7, 1â10 (2017).

mouse

IHC-IF

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anti-Channelrhodopsin 2 mouse monoclonal, 15E2, supernatant

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