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human colon carcinoma (courtesy of L. Langbein) Zoom

human colon carcinoma (courtesy of L. Langbein)

anti-Cingulin guinea pig polyclonal, serum

The anti-cingulin antibody reacts with a 150 kD polypeptide in tight junctions (zonula occludens) of polar epithelia and of stratified epithelia.
Cat. No.: GP26
Quantity:  100 µL

Delivery Time: usually 1-7 working days

€249.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host guinea pig
Antibody Type polyclonal
Immunogen full length GST-fusion protein of human cingulin
Purification stabilized antiserum
Conjugate unconjugated
Formulation contains 0.09% sodium azide and 0.5% BSA
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human, mouse, rabbit, Xenopus laevis

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) 1:100 (initial fixation with formaldehyde recommended for optimal staining)
Immunohistochemistry (IHC) - frozen assay dependent
Immunohistochemistry (IHC) - paraffin assay dependent (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

The antibody reacts specifically with cingulin (a ca.150 kD polypeptide) present in tight junctions (zonula occludens) of polar epithelia (e.g. colon, duodenum, kidney, liver, pancreas) of transitional epithelia (e.g. bladder epithelium) and of stratified epithelia (e.g. skin, foot sole, tongue, oesophagus, vagina).
It is negative with endothelia of vessels and capillaries (e.g. in heart and brain).

Polypeptide reacting: Cingulin, a polypeptide of 126.5 kD (calculated from aa sequence data); apparent Mr 150 kD (after SDS-PAGE); pI 6.5 (very similar to that of symplekin, another junctional protein).

Reactivity on cultured cell lines: Carcinoma cell lines of human(e.g. MCF-7, Caco-2, PLC, A-431), bovine (MDBK, BMGE) and canine (MDCK) origin.

Positive control: total cell lysate of e.g. CaCo-2 cells.

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