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anti-Cingulin mouse monoclonal, 139.3.4, supernatant

The anti-Cingulin antibody reacts with a 150 kD polypeptide in tight junctions (zonula occludens) of polar epithelia and of stratified epithelia.
Cat. No.: 651122
Quantity:  5 mL
No. of experiments:  ≤ 100 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgM
Clone 139.3.4
Immunogen synthetic peptide (aa. 4-24)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments ≤ 100 (IHC, 50 µl/slide)
Intended use Research use only
Tested species reactivity bovine, human, mouse (negative with rabbit)


Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent



The mab reacts specifically with cingulin (a 150 kD polypeptide) present in tight junctions (zonula occludens) of polar epithelia (e.g. colon, duodenum, kidney, liver, pancreas), of transitional epithelia (e.g. bladder epithelium), and of stratified epithelia (e.g. skin, foot sole, tongue, oesophagus, vagina).
Negative with endothelia of vessels and capillaries (e.g. in heart and brain).

Polypeptide reacting: Cingulin, 126.5 kD polypeptide (calculated from aa sequence data); apparent Mr 150 kD (after SDS-PAGE); pI 6.5 (very similar to symplekin, another junctional protein).

Reactivity on cultured cell lines: Carcinoma cell lines (e.g. MCF-7, Caco-2, PLC, A-431), SV80 (fibroblasts), Glioma; MDBK, MDCK, BMGE.


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