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anti-Clathrin mouse monoclonal, CHC 5.9, lyophilized, purified

Marker for receptor-mediated endocytosis. The anti-Clathrin antibody reacts with coated vesicles of different organs and tissues (e.g. brain, mammary gland, ovaries).

Cat. No.: 61017
Quantity:  100 µg

Delivery Time: usually 1-7 working days

€180.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgM
Clone CHC5.9
Immunogen Coated vesicles (clathrin) of bovine brain
Purification size exclusion chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity amphibia, bovine, human, pig, rat
Intended use Research use only

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10
Western Blot (WB) 1:100 - 1:500

Background

Details

CHC5.9 represents an excellent marker for detection of receptor mediated endocytosis.
Reactive polypeptide: Mr 180 000 polypeptide (clathrin heavy chain) from coated vesicles of various tissues.
Structures and tissues specifically detected: Coated vesicles of different organs and tissues (eg brain, mammary gland, ovaries).
Reactivity on cultured cell lines: HeLa, SV-40, RVF-SMC

Reference

Reference

PublicationSpeciesApplication
Bruder, G. & Wiedenmann, B. Identification of a distinct 9S form of soluble clathrin in cultured cells and tissues. Exp. Cell Res. 164, 449–462 (1986). human, rat, bovine WB, ICC-IF, IEM

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