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anti-Collagen Type IV mouse monoclonal, 1042.0, supernatant

Reacts specifically with collagen type IV in the basement membrane; helps to distinguish between non-invasive and invasive lesions. No cross-reactivity with other collagen types.
Cat. No.: 10710
Quantity:  1 mL

Delivery Time: usually 1-7 working days

€259.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2b
Clone 1042
Immunogen isolated from human placenta
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human
Intended use Research use only

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10 – 1:20
Immunohistochemistry (IHC) - paraffin 1:10 – 1:20 (protease (pepsin) treatment and/or microwave treatment recommended)

Background

Details

Antibody 1042 is reactive with human collagen type IV of basement membranes, and shows a homogeneous staining pattern in all tissues. As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antibody is useful to distinguish between non-invasive and invasive lesions. Additionally, it can be used for the differentiation of bullous lesions in dermatopathology.
No crossreactivity with other collagens.

Positive control: Skin, kidney.

Reference

Reference

PublicationSpeciesApplication
Havenith, M. G. et al. Human specific anti-type IV collagen monoclonal antibodies, characterization and immunohistochemical application. Histochemistry 87, 123–128 (1987). human WB, IHC (paraffin), ELISA

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