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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG2b |
| Clone | H13 |
| Immunogen | human complement component C3 |
| Purification | protein A affinity chromatography |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 200 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | guinea pig, human, rabbit |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:10 |
| Western Blot (WB) | assay dependent |
Background
Details
H 13 recognizes a C3a-determinant (eg C3 alpha-chain) which is present also on native C3. H 13 allows discrimination of ubiquitous or nonspecifically bound native C3 from biologically active C3b fragment; C3b or C are negative with H 13
Polypeptide reacting: C3a-determinant on C3 alpha-chain
Specific Application: Detection of C3 deposits in tissue, cells, microorganisms and immune complexes. It should be used with positive control anti-C3b (H11/H206). Non-activated, absorbed C3 contains the C3a determinant, whereas activated C3 is negative with H 13.
