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anti-Complement C3b alpha mouse monoclonal, H206, lyophilized, purified

Recognizes human complement component C3b alpha. H206 allows demonstration of C3 deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d.
Cat. No.: 61019
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€201.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone H206
Immunogen human complement component C3
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human
Intended use Research use only

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunohistochemistry (IHC) - frozen 1:10
Western Blot (WB) assay dependent

Background

Details

H 206 allows demonstration of C3 deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d and does not inhibit hemolytic function of C3. The epitope was located on the C-termius of the alpha-chain (39.5 kD fragment of C3c).

Disorders specifically detected: Detection of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It should be used with positive control anti C3b-ß, (H11; Cat. No. 61020) and negative control anti-C3a (H13; Cat. No. 61018). Activated, deposited C3b bears C3b-a and C3b-ß, but no longer C3a. Ubiquitous or nonspecifically absorbed C3 is still C3a positive with H 13.

Polypeptide reacting: C3b alpha-chain.

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