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anti-Complement C3b beta mouse monoclonal, H11, lyophilized, purified

Recognizes human complement component C3b beta. H11 allows demonstration of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d.
Cat. No.: 61020
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€211.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone H11
Immunogen human complement component C3
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
UniprotID P01024
Intended use Research use only
Tested species reactivity human

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10
Western Blot (WB) assay dependent

Background

Details

H 11 allows demonstration of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d; inhibits hemolytic function of C3. H 11 specifically reacts with C3b beta-chain.

Detection of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It should be used with positive control anti C3b-α (H206), and negative control anti C3a (H13). Activated, deposited C3b bears 3b-α and -ß, but no longer C3a. Ubiquitous or non-specifically absorbed C3 is still C3a positive with H 13.

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