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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | H11 |
| Immunogen | human complement component C3 |
| Purification | protein A affinity chromatography |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| UniprotID | P01024 |
| No. of experiments | ≤ 200 (IHC, 50 µl/slide) |
| Intended use | Research use only |
| Tested species reactivity | human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:10 |
| Western Blot (WB) | assay dependent |
Background
Details
H 11 allows demonstration of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d; inhibits hemolytic function of C3. H 11 specifically reacts with C3b beta-chain.
Detection of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It should be used with positive control anti C3b-α (H206), and negative control anti C3a (H13). Activated, deposited C3b bears 3b-α and -ß, but no longer C3a. Ubiquitous or non-specifically absorbed C3 is still C3a positive with H 13.
Reference
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