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Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | HCC5.1 |
| Immunogen | human complement component C5 |
| Purification | protein A affinity chromatography |
| Conjugate | unconjugated |
| Formulation | lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
| Storage | short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles |
| Intended use | Research use only |
| Tested species reactivity | human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| ELISA | assay dependent |
| Immunohistochemistry (IHC) - frozen | 1:10 |
| Western Blot (WB) | assay dependent |
Background
Details
HCC 5.1 allows detection of native complement component C5 and discrimination from activated C5b-9 complexes, especially in different forms of glomerulonephritis.
Polypeptide reacting: Mr 200,000 polypeptide of native complement component C5.
Disorders specifically detected: HCC 5.1 reacts with native C5 as ell as with C5b. Native C5 is only detected with HCC 5.1, while C5b is detected by both HCC 5.1 and HCC 5.2.
Reference
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