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anti-Complement C5b neo-Epitope mouse monoclonal, HCC5b.1, lyophilized, purified

HCC5b.1(neo) allows detection of C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis. Does not react with native C5.
Cat. No.: 61022
Quantity:  50 µg

Delivery Time: usually 1-7 working days

$251.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone HCC5b.1(neo)
Immunogen activated human complement component C5
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity bovine, goat, human, pig, primates

Applications

Tested applications Tested dilutions
ELISA assay dependent
Immunohistochemistry (IHC) - frozen 1:10
Western Blot (WB) assay dependent

Background

Details

HCC 5b.1(neo) allows detection of C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis. Does not react with native C5!
Polypeptide reacting: Mr 200 000 polypeptide of SDS denaturated complement component C5 and C5b of Mr 185 000; after C5b activation, an Mr 60 000 cleavage product also reacts (weakly)
Disorders specifically detected: Detection of C5b-9 complexes via activated C5, whereas native C5 reacts with antibody HCC 5.1

Reference

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