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anti-Cyclin D1 mouse monoclonal, DCS-6, lyophilized, purified

The monoclonal DCS-6 anti-cyclin D1 antibody reacts specifically with cyclin D1 protein present predominantly in G1 phase of cell cycle; does not cross-react with D2 or D3 cyclins. The anti-cyclin D1 antibody is suitable as a fast method to identify cases with 11q13 amplifications.
Cat. No.: 61053
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€160.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG2a
Clone DCS-6
Immunogen human recombinant full-length cyclin D1 protein
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity dog, human, monkey, mouse, rat

Applications

Tested applications Tested dilutions
Flow Cytometry (FACS) assay dependent
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:10
Immunohistochemistry (IHC) - paraffin 1:10 (microwave treatment recommended)
Immunoprecipitation (IP) assay dependent
Neutralization Assay assay dependent
Western Blot (WB) assay dependent

Background

Details

DCS-6 reacts specifically with cyclin D1 protein present predominantly in G1 phase of cell cycle; it does not cross-react with D2 or D3 cyclins (the closest relatives within the cyclin family); DCS-6 is especially useful in tumor diagnosis: it can be used as fast method to identify cases with 11q13 amplifications. Cyclin D1 gene is identical to the BCL-1 and the PRAD-1 oncogene and is part of the chromosome 11q13 amplicon which is amplified in several common tumor types like breast, lung, esophagus, urinary bladder and head and neck carcinoma. Polypeptide reacting: Mr 36 000 polypeptide (cyclin D1 polypeptide of human cells).

Reactivity on cultured cell lines: MCF-7, MDA-MB-134 (breast cancer cell lines), UMSC22 (squamous cell carcinoma cell line), U-2-OS (osteosarcoma cell line) RT 112, A 431, HT 29.

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