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anti-Cyclin-Dependent Kinase 4 mouse monoclonal, DCS-156, lyophilized, purified

The anti-Cyclin-Dependent Kinase 4 (Cdk4) antibody reacts specifically with cdk4 protein present predominantly in G1 phase of cell cycle; it does not cross-react with cyclins. On frozen sections of head and neck carcinoma (oropharynx and hypopharynx carcinoma) the anti-Cyclin-Dependent Kinase 4 monoclonal DCS-156 antibody shows distinct nuclear staining.
Cat. No.: 61096
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€160.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone DCS-156
Immunogen human recombinant full-length cdk4 polypeptide
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human, mouse, rat

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10
Immunohistochemistry (IHC) - paraffin 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

DCS-156 reacts specifically with cdk4 protein present predominantly in G1 phase of cell cycle; it does not cross-react with cyclins. In immunohistochemical application on frozen sections of head and neck carcinoma (oropharynx and hypopharynx carcinoma) and cervix DCS-156 shows distinct nuclear staining, especially in tumor areas of enhanced proliferation.
Epitope recognized: aa 270-290.

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