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anti-Cyclin-Dependent Kinase 4 mouse monoclonal, DCS-156, supernatant

The anti-Cyclin-Dependent Kinase 4 (Cdk4) antibody reacts specifically with cdk4 protein present predominantly in G1 phase of cell cycle; it does not cross-react with cyclins. On frozen sections of head and neck carcinoma (oropharynx and hypopharynx carcinoma) The anti-Cyclin-Dependent Kinase 4 monoclonal DCS-156 antibody shows distinct nuclear staining.
Cat. No.: 65196
Quantity:  5 mL

Delivery Time: usually 1-7 working days

€129.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone DCS-156
Immunogen human recombinant full-length cdk4 polypeptide
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

DCS-156 reacts specifically with cdk4 protein present predominantly in G1 phase of cell cycle; it does not cross-react with cyclins. In immunohistochemi-cal application on frozen sections of head and neck carcinoma (oropharynx and hypopharynx carcinoma) DCS-156 shows distinct nuclear staining, especially in tumor areas of enhanced proliferation.

Reference

Reference

Wistuba A.,Weger S., Kern A., and Kleinschmidt J. (1995) Intermediates of adeno-associated virus type 2 assembly: Identifica-tion of soluble complexes containing Rep and Cap proteins. J. Virol. 69, 5311-5319.

Mietzsch M, Grasse S, Zurawski C, Weger S, Bennett A, Agbandje-McKenna M, Muzyczka N, Zolotukhin S, Heilbronn R: One-Bac: Platform for scalable and high-titer production of adeno-associated virus serotype 1-12 vectors for gene therapy. Hum Gene Ther 25, 212-222 (2014); doi: 10.1089/hum.2013.184

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