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anti-Cylicin I mouse monoclonal, Cal-144, supernatant

Especially useful in discrimination of morphologically altered "round-headed" spermatozoa.
Cat. No.: 65142
Quantity:  5 mL

Delivery Time: usually 1-7 working days

$174.00
Excl. 19% Tax, excl. Shipping Cost

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Specifications

Product description

Host mouse
Antibody Type monoclonal
Isotype IgM
Clone Cal-144 (X 144.3.2)
Immunogen calyx preparation from bovine sperm (according to Longo et al. 1987, JCB 22, 1105-1120)
Purification hybridoma cell culture supernatant
Conjugate unconjugated
Formulation contains 0.09% sodium azide
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity bovine, human

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) ready-to-use
Western Blot (WB) assay dependent

Background

Details

Especially useful in discrimination of morphologically altered "round-headed" spermatozoa.

Polypeptides reacting: Bovine cylicin I (Mr 74.788, deduced from sequence analysis), a basic protein (IEP 10.55) of  the sperm head cytoskeleton with Mr 58.000 on SDS-PAGE; Multiple band proteins with MW 58, 63, 69 and 74 kDa of calyx-associated polypeptides of bovine spermatozoa;

Reference

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