Product description
Host | guinea pig |
---|---|
Antibody Type | polyclonal |
Immunogen | synthetic peptide J7 (corresponding to aa 1175-1192), coupled to KLH |
Purification | stabilized antiserum |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide and 0.5% BSA |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 200 (IHC, 50 µl/slide); ≤ 50 (WB, 5 ml reaction volume) |
Note | centrifuge prior to opening |
Intended use | Research use only |
Tested species reactivity | bovine, human, mouse, rat |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:100 |
Immunohistochemistry (IHC) - frozen | 1:100 |
Immunohistochemistry (IHC) - paraffin | 1:100 (microwave treatment recommended) |
Western Blot (WB) | 1:2,500 |
Background
Details
The antibody reacts specifically with neurojungin (delta-catenin, a unique 160 kD polypeptide of the plakoglobin / armadillo protein family) present in adhering type junctions in the nervous system, primarily expressed in the brain. Delta-catenin also binds to presenilin-1, mutations of this protein are described to play a role in familial Alzheimer’s disease. No cross-reaction with other members of the armadillo / plakoglobin protein family.
Polypeptide reacting: Neurojungin (delta-catenin), 160 kD polypeptide.
Reactivity on cultured cell lines: Murine cell line A1B2, stably transfected with neurojungin cDNA.
Positive control: Murine brain, retina.
Reference
Filter