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anti-Desmocollin 1 mouse monoclonal, Dsc1-U100, lyophilized, purified

The anti-desmocollin 1 antibody localizes desmocollin 1 in suprabasal layers of interfollicular epidermis, specific cell layers, e.g. hair follicle root sheat, and Hassall bodies.
Cat. No.: 61092
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€234.00
Excl. 19% Tax, excl. Shipping Cost

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Dsc1-U100
Immunogen synthetic peptide corresponding to sequence present within the intracellular part of human desmocollin 1 (HQTLESVKGVGQGDTGRYATDWQC)
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 mL dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human (negative with bovine and rodents)

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10 (preincubation (directly after fixation) with 0.05 - 0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended; without detergent pretreatment a cytoplasmic component might cross-react)
Immunohistochemistry (IHC) - paraffin 1:10 (microwave treatment recommended; a slight crossreactivity with a lymphocyte subpopulation is abolished by mild trypsinization after the microwave step)
Western Blot (WB) assay dependent

Background

Details

Mab localizes Dsc1 present in

  • suprabasal layers of interfollicular epidermis
  • specific cell layers (e.g. in hair follicle root sheath)
  • Hassall bodies (spheroidal formations of densely packed reticulum-derived cells in the thymus).

Reacts with 111 kD (form "a") and 103 kD (form "b") polypeptides (desmo-collin 1 splice forms, both at pI 5.2) from human epidermal desmosomes.

Reactivity on cultured cell lines: Positive on primary keratinocytes, however, restricted to local piles of differentiated cells. All simple epithelia are negative.

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