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anti-Desmocollin 3 mouse monoclonal, Dsc3-U114, lyophilized, purified

The anti-desmocollin 3 antibody localizes desmocollin 3 in living epidermal layers, glandular ducts cells, basal matrix cells, basal and suprabasal layers of stratified epithelia, and thymic reticulum cells. All simple epithelia are negative.
Cat. No.: 61093
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€234.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone Dsc3-U114
Immunogen Synthetic peptide corresponding to a sequence present on the extracellular anchor domain of human desmocollin 3 (VHGAPFYFSLPNTSPEISRLWSLTKVC)
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity human (negative with bovine and rodent)

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen 1:10 (for better resolution preincubation (directly after fixation) with 0.05 - 0.2% Triton X100, for 5-10 min, depending on tissue type, is recommended )
Immunohistochemistry (IHC) - paraffin 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

Mab localizes Dsc3 present in

  • all living epidermal layers
  • in glandular duct cells
  • in basal matrix cells and outer root sheath of hair follicles
  • basal and suprabasal cell layers of stratified epithelia (e.g. vagina, tongue, esophagus), basal layer of bladder urothelium and complex epithelium of trachea
  • thymic reticulum cells

All simple epithelia are negative. Intercalated disks of myocardium are
negative. Polypeptide reacting: 109 kD and 100 kD polypeptides (desmocollin 3 splice isoforms at pI 5.2) from human epidermal desmosomes.
Reactivity on cultured cell lines: Most cultured cell lines derived from stratified squamous epithelia or squamous cell carcinomas are positive (e.g. A-431, HaCaT).

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