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anti-Desmoglein 1/2 mouse monoclonal, DG 3.10, lyophilized, purified

DG 3.10 anti-desmoglein 1/2 antibody recognizes desmoglein 1 + 2 in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type.

Cat. No.: 61002
Quantity:  50 µg

Delivery Time: usually 1-7 working days

€201.00
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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone DG 3.10
Immunogen "Band 3" polypeptide of isolated desmosomes from bovine muzzle epidermis
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 mL dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human, mouse, rat

Applications

Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen at least 1:10
Immunohistochemistry (IHC) - paraffin at least 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent

Background

Details

DG 3.10 recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide reacting: Mr 165 000 Desmoglein, "band 3" polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide.

Reactivity on cultured cell lines: A-431, MCF-7, TR-146, PLC and various other human epithelial cell lines; bovine BMGE+H, BMGE-H, MDBK.

Reference

Reference

  1. Crespi, A. et al. POF1B Localizes to Desmosomes and Regulates Cell Adhesion in Human Intestinal and Keratinocyte Cell Lines. J. Invest. Dermatol. 135, 192–201 (2015).
  2. Izawa, G., Kobayashi, W., Haraguchi, M., Sudo, A. & Ozawa, M. The ectopic expression of Snail in MDBK cells does not induce epithelial-mesenchymal transition. Int. J. Mol. Med. 36, 166–72 (2015).
  3. Noritake, K., Aki, T., Funakoshi, T., Unuma, K. & Uemura, K. Direct Exposure to Ethanol Disrupts Junctional Cell-Cell Contact and Hippo-YAP Signaling in HL-1 Murine Atrial Cardiomyocytes. PLoS One (2015). doi:10.1371/journal
  4. Jennemann, R. et al. Loss of ceramide synthase 3 causes lethal skin barrier disruption. Hum. Mol. Genet. 21, 586–608 (2012).
  5. Lombardi, R. et al. Nuclear plakoglobin is essential for differentiation of cardiac progenitor cells to adipocytes in arrhythmogenic right ventricular cardiomyopathy. Circ. Res. 109, 1342–53 (2011).
  6. Bosch, F. X., Andl, C., Abel, U. & Kartenbeck, J. E-cadherin is a selective and strongly dominant prognostic factor in squamous cell carcinoma: a comparison of E-cadherin with desmosomal components. Int. J. cancer 114, 779–90 (2005).
  7. Kartenbeck, J., Haselmann, U. & Gassler, N. Synthesis of junctional proteins in metastasizing colon cancer cells. Eur. J. Cell Biol. 84, 417–30 (2005).
  8. Akat, K. et al. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 106, 337–47 (2003).
  9. Caldelari, R. et al. A Central Role for the Armadillo Protein Plakoglobin in the Autoimmune Disease Pemphigus Vulgaris. J. Cell Biol. 153, 823–834 (2001).
  10. Hatzfeld, M., Haffner, C., Schulze, K. & Vinzens, U. The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament Organization. J. Cell Biol. 149, 209–222 (2000).
  11. Schäfer, S., Stumpp, S. & Franke, W. W. Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation. 60, 99–108 (1996).
  12. Schäfer, S., Koch, P. J. & Franke, W. W. Identification of the ubiquitous human desmoglein, Dsg2, and the expression catalogue of the desmoglein subfamily of desmosomal cadherins. Exp. Cell Res. 211, 391–9 (1994).
  13. Schmelz, M. & Franke, W. W. Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes. Eur. J. Cell Biol. 61, 274–89 (1993).
  14. Wheeler, G. N. et al. Desmosomal glycoprotein DGI, a component of intercellular desmosome junctions, is related to the cadherin family of cell adhesion molecules (desmosome/desmoglein/ceD-cell junction). Cell Biol. 88, 4796–4800 (1991).
  15. Koch, P. J. et al. Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules. Eur. J. Cell Biol. 53, 1–12 (1990).
  16. Schmelz, M., Duden, R., Cowin, P. & Franke, W. W. A constitutive transmembrane glycoprotein of Mr 165,000 (desmoglein) in epidermal and non-epidermal desmosomes. II. Immunolocalization and microinjection studies. Eur. J. Cell Biol. 42, 184–99 (1986).

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