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anti-Desmoglein 1/2 mouse monoclonal, DG 3.10, lyophilized, purified

DG 3.10 anti-desmoglein 1/2 antibody recognizes desmoglein 1 + 2 in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type.

Cat. No.: 61002
Quantity:  50 µg

Delivery Time: usually 1-7 working days

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Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone DG 3.10
Immunogen "Band 3" polypeptide of isolated desmosomes from bovine muzzle epidermis
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 mL dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Tested species reactivity bovine, human, mouse, rat


Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen at least 1:10
Immunohistochemistry (IHC) - paraffin at least 1:10 (microwave treatment recommended)
Western Blot (WB) assay dependent



DG 3.10 recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide reacting: Mr 165 000 Desmoglein, "band 3" polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide.

Reactivity on cultured cell lines: A-431, MCF-7, TR-146, PLC and various other human epithelial cell lines; bovine BMGE+H, BMGE-H, MDBK.



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  2. Izawa, G., Kobayashi, W., Haraguchi, M., Sudo, A. & Ozawa, M. The ectopic expression of Snail in MDBK cells does not induce epithelial-mesenchymal transition. Int. J. Mol. Med. 36, 166–72 (2015).
  3. Noritake, K., Aki, T., Funakoshi, T., Unuma, K. & Uemura, K. Direct Exposure to Ethanol Disrupts Junctional Cell-Cell Contact and Hippo-YAP Signaling in HL-1 Murine Atrial Cardiomyocytes. PLoS One (2015). doi:10.1371/journal
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  7. Kartenbeck, J., Haselmann, U. & Gassler, N. Synthesis of junctional proteins in metastasizing colon cancer cells. Eur. J. Cell Biol. 84, 417–30 (2005).
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  9. Caldelari, R. et al. A Central Role for the Armadillo Protein Plakoglobin in the Autoimmune Disease Pemphigus Vulgaris. J. Cell Biol. 153, 823–834 (2001).
  10. Hatzfeld, M., Haffner, C., Schulze, K. & Vinzens, U. The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament Organization. J. Cell Biol. 149, 209–222 (2000).
  11. Schäfer, S., Stumpp, S. & Franke, W. W. Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation. 60, 99–108 (1996).
  12. Schäfer, S., Koch, P. J. & Franke, W. W. Identification of the ubiquitous human desmoglein, Dsg2, and the expression catalogue of the desmoglein subfamily of desmosomal cadherins. Exp. Cell Res. 211, 391–9 (1994).
  13. Schmelz, M. & Franke, W. W. Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes. Eur. J. Cell Biol. 61, 274–89 (1993).
  14. Wheeler, G. N. et al. Desmosomal glycoprotein DGI, a component of intercellular desmosome junctions, is related to the cadherin family of cell adhesion molecules (desmosome/desmoglein/ceD-cell junction). Cell Biol. 88, 4796–4800 (1991).
  15. Koch, P. J. et al. Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules. Eur. J. Cell Biol. 53, 1–12 (1990).
  16. Schmelz, M., Duden, R., Cowin, P. & Franke, W. W. A constitutive transmembrane glycoprotein of Mr 165,000 (desmoglein) in epidermal and non-epidermal desmosomes. II. Immunolocalization and microinjection studies. Eur. J. Cell Biol. 42, 184–99 (1986).

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