Call +49 6221 8278-0

PROGEN - passion for research


IHC of human skin (© PROGEN in cooperation with Uniklinik Heidelberg, Dr. Jochen Heß) Zoom

IHC of human skin (© PROGEN in cooperation with Uniklinik Heidelberg, Dr. Jochen Heß)

anti-Desmoglein 1/2 mouse monoclonal, DG 3.10, lyophilized, purified

DG 3.10 anti-desmoglein 1/2 antibody recognizes desmoglein 1 + 2 in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type.

highly_published external_validation
Cat. No.: 61002
Quantity:  50 µg

Delivery Time: usually 1-7 working days

Excl. 19% Tax, excl. Shipping Cost

Call us now for more information on our products: +49 6221 8278-0

Any more questions?


Alternative Products

Cat. No. Product Name Format Quantity Price

anti-Desmosome sample set 2

600 µl each antibody

anti-Desmoglein 1/2 mouse monoclonal, DG 3.10

purified, sample (ready-to-use) 600 µl (ready-to-use)
Excl. 19% Tax, excl. Shipping Cost

Product description

Host mouse
Antibody Type monoclonal
Isotype IgG1
Clone DG 3.10
Immunogen "Band 3" polypeptide of isolated desmosomes from bovine muzzle epidermis
Purification protein A affinity chromatography
Conjugate unconjugated
Formulation lyophilized; reconstitute in 1 mL dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Intended use Research use only
Tested species reactivity bovine, human, rat


Tested applications Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
Immunohistochemistry (IHC) - frozen 1:10 - 1:100
Immunohistochemistry (IHC) - paraffin 1:10 - 1:100 (microwave treatment recommended)
Western Blot (WB) assay dependent



DG 3.10 recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide reacting: Mr 165 000 Desmoglein, "band 3" polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide.

Reactivity on cultured cell lines: A-431, MDBK.



Price, A. et al. Mechanical loading of desmosomes depends on the magnitude and orientation of external stress. Nat.Commun. 9, 5284 (2018). mouse ICC-IF
Izawa, G., Kobayashi, W., Haraguchi, M., Sudo, A. & Ozawa, M. The ectopic expression of Snail in MDBK cells does not induce epithelial-mesenchymal transition. Int. J. Mol. Med. 36, 166–72 (2015). bovine WB
Noritake, K., Aki, T., Funakoshi, T., Unuma, K. & Uemura, K. Direct Exposure to Ethanol Disrupts Junctional Cell-Cell Contact and Hippo-YAP Signaling in HL-1 Murine Atrial Cardiomyocytes. PLoS One (2015). mouse WB
Jennemann, R. et al. Loss of ceramide synthase 3 causes lethal skin barrier disruption. Hum. Mol. Genet. 21, 586–608 (2012). mouse WB, IHC (paraffin)
Lombardi, R. et al. Nuclear plakoglobin is essential for differentiation of cardiac progenitor cells to adipocytes in arrhythmogenic right ventricular cardiomyopathy. Circ. Res. 109, 1342–53 (2011). mouse Co-IP
Rickelt, S., Winter-Simanowski, S., Noffz, E., Kuhn, C. & Franke, W. W. Upregulation of plakophilin-2 and its acquisition to adherens junctions identifies a novel molecular ensemble of cell-cell-attachment characteristic for transformed mesenchymal cells. Int. J. cancer 125, 2036–48 (2009). human WB
Pieperhoff, S. & Franke, W. W. The area composita of adhering junctions connecting heart muscle cells of vertebrates – IV: Coalescence and amalgamation of desmosomal and adhaerens junction components – Late processes in mammalian heart development. Eur. J. Cell Biol. 86, 377–391 (2007). mouse IHC (frozen)
Borrmann, C. M. et al. The area composita of adhering junctions connecting heart muscle cells of vertebrates. II. Colocalizations of desmosomal and fascia adhaerens molecules in the intercalated disk. Eur. J. Cell Biol. 85, 469–485 (2006). human IHC (frozen)
Franke, W. W., Borrmann, C. M., Grund, C. & Pieperhoff, S. The area composita of adhering junctions connecting heart muscle cells of vertebrates. I. Molecular definition in intercalated disks of cardiomyocytes by immunoelectron microscopy of desmosomal proteins. European Journal of Cell Biology 85, 69–82 (2006). human IEM
Bosch, F. X., Andl, C., Abel, U. & Kartenbeck, J. E-cadherin is a selective and strongly dominant prognostic factor in squamous cell carcinoma: a comparison of E-cadherin with desmosomal components. Int. J. cancer 114, 779–90 (2005). human IHC (frozen)
Kartenbeck, J., Haselmann, U. & Gassler, N. Synthesis of junctional proteins in metastasizing colon cancer cells. Eur. J. Cell Biol. 84, 417–30 (2005). human IHC (paraffin), IEM
Peitsch, W. K. et al. Drebrin, an Actin-Binding, Cell-Type Characteristic Protein: Induction and Localization in Epithelial Skin Tumors and Cultured Keratinocytes. J. Invest. Dermatol. 125, 761–774 (2005). human IHC (frozen)
Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114–30 (2003). human WB, ICC-IF
Caldelari, R. et al. A Central Role for the Armadillo Protein Plakoglobin in the Autoimmune Disease Pemphigus Vulgaris. J. Cell Biol. 153, 823–834 (2001). mouse WB
Hatzfeld, M., Haffner, C., Schulze, K. & Vinzens, U. The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament Organization. J. Cell Biol. 149, 209–222 (2000). human ICC-IF
Schäfer, S., Stumpp, S. & Franke, W. W. Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation. 60, 99–108 (1996). human WB
Chitaev, N. A. et al. The Binding of Plakoglobin to Desmosomal Cadherins: Patterns of Binding Sites and Topogenic Potential. J. Cell Biol. 113, 359–369 (1996). human WB, ICC-IF
Leube, R. E. The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains. J. Cell Sci. 108, 883–894 (1995). human ICC-IF
Schmelz, M. & Franke, W. W. Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes. Eur. J. Cell Biol. 61, 274–89 (1993). human, rat, bovine WB, IHC (frozen), ICC-IF, IEM

Material safety data sheet

Please complete the form below to request a material safety data sheet

Personal Info

* Required Fields

Product Request

Personal Info

* Required Fields