IHC of human skin (© PROGEN in cooperation with Uniklinik Heidelberg, Dr. Jochen Heß)

anti-Desmoglein 1/2 mouse monoclonal, DG 3.10, lyophilized, purified

DG 3.10 anti-desmoglein 1/2 antibody recognizes desmoglein 1 + 2 in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type.

Cat. No.: 61002
Quantity: 50 µg
No. of experiments: ≤ 2000 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$318.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Schinner, C. et al. Stabilization of desmoglein-2 binding rescues arrhythmia in arrhythmogenic cardiomyopathy. JCI.Insight. 5, (2020)

mouse

WB
IHC (frozen)

Domke, L and Franke, W. The cell-cell junctions of mammalian testes.., Cell Tissue Res, 375, 451-482, (2019)

pig

ICC-IF

Ding, X. et al. Epidermal mammalian target of rapamycin complex 2 controls lipid synthesis and filaggrin processing in epidermal barrier formation. J.Allergy.Clin.Immunol. , (2019)

mouse

Imanishi, I. et al. Exfoliative toxin E, a new Staphylococcus aureus virulence factor with host-specific activity. Sci.Rep. 9, 16336 (2019)

mouse

IHC (frozen)

Price, A. et al. Mechanical loading of desmosomes depends on theÂ magnitude and orientation of external stress. Nat.Commun. 9, 5284 (2018).

mouse

DedeiÄ, Z. et al. Cell autonomous role of iASPP deficiency in causing cardiocutaneous disorders. Cell.Death.Differ. 25, 1289-1303 (2018)

mouse

ICC-IF

Izawa, G., Kobayashi, W., Haraguchi, M., Sudo, A. & Ozawa, M. The ectopic expression of Snail in MDBK cells does not induce epithelial-mesenchymal transition. Int. J. Mol. Med. 36, 166â72 (2015).

bovine

Noritake, K., Aki, T., Funakoshi, T., Unuma, K. & Uemura, K. Direct Exposure to Ethanol Disrupts Junctional Cell-Cell Contact and Hippo-YAP Signaling in HL-1 Murine Atrial Cardiomyocytes. PLoS One (2015).

mouse

WB
IHC (paraffin)

Jennemann, R. et al. Loss of ceramide synthase 3 causes lethal skin barrier disruption. Hum. Mol. Genet. 21, 586â608 (2012).

mouse

Co-IP

Lombardi, R. et al. Nuclear plakoglobin is essential for differentiation of cardiac progenitor cells to adipocytes in arrhythmogenic right ventricular cardiomyopathy. Circ. Res. 109, 1342â53 (2011).

mouse

Rickelt, S., Winter-Simanowski, S., Noffz, E., Kuhn, C. & Franke, W. W. Upregulation of plakophilin-2 and its acquisition to adherens junctions identifies a novel molecular ensemble of cell-cell-attachment characteristic for transformed mesenchymal cells.

human

IHC (frozen)

Pieperhoff, S. & Franke, W. W. The area composita of adhering junctions connecting heart muscle cells of vertebrates â IV: Coalescence and amalgamation of desmosomal and adhaerens junction components â Late processes in mammalian heart development. Eur. J

mouse

IHC (frozen)

Borrmann, C. M. et al. The area composita of adhering junctions connecting heart muscle cells of vertebrates. II. Colocalizations of desmosomal and fascia adhaerens molecules in the intercalated disk. Eur. J. Cell Biol. 85, 469â485 (2006).

human

IEM

Franke, W. W., Borrmann, C. M., Grund, C. & Pieperhoff, S. The area composita of adhering junctions connecting heart muscle cells of vertebrates. I. Molecular definition in intercalated disks of cardiomyocytes by immunoelectron microscopy of desmosomal pr

human

IHC (frozen)

Bosch, F. X., Andl, C., Abel, U. & Kartenbeck, J. E-cadherin is a selective and strongly dominant prognostic factor in squamous cell carcinoma: a comparison of E-cadherin with desmosomal components. Int. J. cancer 114, 779â90 (2005).

human

IHC (paraffin)
IEM

Kartenbeck, J., Haselmann, U. & Gassler, N. Synthesis of junctional proteins in metastasizing colon cancer cells. Eur. J. Cell Biol. 84, 417â30 (2005).

human

IHC (frozen)

Peitsch, W. K. et al. Drebrin, an Actin-Binding, Cell-Type Characteristic Protein: Induction and Localization in Epithelial Skin Tumors and Cultured Keratinocytes. J. Invest. Dermatol. 125, 761â774 (2005).

human

WB
ICC-IF

Koeser, J., Troyanovsky, S. M., Grund, C. & Franke, W. W. De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components. Exp. Cell Res. 285, 114â30 (2003).

human

Caldelari, R. et al. A Central Role for the Armadillo Protein Plakoglobin in the Autoimmune Disease Pemphigus Vulgaris. J. Cell Biol. 153, 823â834 (2001).

mouse

WB
ICC-IF

Hatzfeld, M., Haffner, C., Schulze, K. & Vinzens, U. The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament Organization. J. Cell Biol. 149, 209â222 (2000).

human

ICC-IF

SchÃ¤fer, S., Stumpp, S. & Franke, W. W. Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation. 60, 99â108 (1996).

human

Chitaev, N. A. et al. The Binding of Plakoglobin to Desmosomal Cadherins: Patterns of Binding Sites and Topogenic Potential. J. Cell Biol. 113, 359â369 (1996).

human

WB
ICC-IF

Leube, R. E. The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains. J. Cell Sci. 108, 883â894 (1995).

human

ICC-IF

Schmelz, M. & Franke, W. W. Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes. Eur. J. Cell Biol. 61, 274â89 (1993).

human
rat
bovine

WB
IHC (frozen)
ICC-IF
IEM

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
70031	anti-Desmosome sample set 2		600 µl each antibody	$184.00
61002S	anti-Desmoglein 1/2 mouse monoclonal, DG 3.10	purified, sample (ready-to-use)	600 µl (ready-to-use)	$65.00 Excl. VAT, excl. Shipping Cost

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG1
Clone	DG 3.10
Immunogen	"Band 3" polypeptide of isolated desmosomes from bovine muzzle epidermis
Purification	protein A affinity chromatography
Conjugate	unconjugated
Formulation	lyophilized; reconstitute in 1 mL dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments	≤ 2000 (IHC, 50 µl/slide)
Intended use	Research use only
Tested species reactivity	bovine, human, rat

Applications

Tested applications	Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	assay dependent
Immunohistochemistry (IHC) - frozen	1:10 - 1:100
Immunohistochemistry (IHC) - paraffin	1:10 - 1:100 (microwave treatment recommended)
Western Blot (WB)	assay dependent

Background

Details

DG 3.10 recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide reacting: Mr 165 000 Desmoglein, "band 3" polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide.

Reactivity on cultured cell lines: A-431, MDBK.

Reference

Publication

Species

Application

Schinner, C. et al. Stabilization of desmoglein-2 binding rescues arrhythmia in arrhythmogenic cardiomyopathy. JCI.Insight. 5, (2020)

mouse

WB
IHC (frozen)

Domke, L and Franke, W. The cell-cell junctions of mammalian testes.., Cell Tissue Res, 375, 451-482, (2019)

pig

ICC-IF

Ding, X. et al. Epidermal mammalian target of rapamycin complex 2 controls lipid synthesis and filaggrin processing in epidermal barrier formation. J.Allergy.Clin.Immunol. , (2019)

mouse

Imanishi, I. et al. Exfoliative toxin E, a new Staphylococcus aureus virulence factor with host-specific activity. Sci.Rep. 9, 16336 (2019)

mouse

IHC (frozen)

Price, A. et al. Mechanical loading of desmosomes depends on theÂ magnitude and orientation of external stress. Nat.Commun. 9, 5284 (2018).

mouse

DedeiÄ, Z. et al. Cell autonomous role of iASPP deficiency in causing cardiocutaneous disorders. Cell.Death.Differ. 25, 1289-1303 (2018)

mouse

ICC-IF

Izawa, G., Kobayashi, W., Haraguchi, M., Sudo, A. & Ozawa, M. The ectopic expression of Snail in MDBK cells does not induce epithelial-mesenchymal transition. Int. J. Mol. Med. 36, 166â72 (2015).

bovine

mouse

WB
IHC (paraffin)

Jennemann, R. et al. Loss of ceramide synthase 3 causes lethal skin barrier disruption. Hum. Mol. Genet. 21, 586â608 (2012).

mouse

Co-IP

Lombardi, R. et al. Nuclear plakoglobin is essential for differentiation of cardiac progenitor cells to adipocytes in arrhythmogenic right ventricular cardiomyopathy. Circ. Res. 109, 1342â53 (2011).

mouse

human

IHC (frozen)

mouse

IHC (frozen)

human

IEM

human

IHC (frozen)

human

IHC (paraffin)
IEM

Kartenbeck, J., Haselmann, U. & Gassler, N. Synthesis of junctional proteins in metastasizing colon cancer cells. Eur. J. Cell Biol. 84, 417â30 (2005).

human

IHC (frozen)

human

WB
ICC-IF

human

Caldelari, R. et al. A Central Role for the Armadillo Protein Plakoglobin in the Autoimmune Disease Pemphigus Vulgaris. J. Cell Biol. 153, 823â834 (2001).

mouse

WB
ICC-IF

Hatzfeld, M., Haffner, C., Schulze, K. & Vinzens, U. The Function of Plakophilin 1 in Desmosome Assembly and Actin Filament Organization. J. Cell Biol. 149, 209â222 (2000).

human

ICC-IF

human

Chitaev, N. A. et al. The Binding of Plakoglobin to Desmosomal Cadherins: Patterns of Binding Sites and Topogenic Potential. J. Cell Biol. 113, 359â369 (1996).

human

WB
ICC-IF

Leube, R. E. The topogenic fate of the polytopic transmembrane proteins, synaptophysin and connexin, is determined by their membrane-spanning domains. J. Cell Sci. 108, 883â894 (1995).

human

ICC-IF

human
rat
bovine

WB
IHC (frozen)
ICC-IF
IEM

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anti-Desmoglein 1/2 mouse monoclonal, DG 3.10, lyophilized, purified

Alternative Products

anti-Desmosome sample set 2