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Alternative Products
Cat. No. | Product Name | Format | Quantity | Price |
---|---|---|---|---|
70031 |
anti-Desmosome sample set 2 |
600 µl each antibody |
$184.00
|
|
61002S |
anti-Desmoglein 1/2 mouse monoclonal, DG 3.10 |
purified, sample (ready-to-use) | 600 µl (ready-to-use) |
$65.00
Excl. VAT, excl. Shipping Cost
|
Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG1 |
Clone | DG 3.10 |
Immunogen | "Band 3" polypeptide of isolated desmosomes from bovine muzzle epidermis |
Purification | protein A affinity chromatography |
Conjugate | unconjugated |
Formulation | lyophilized; reconstitute in 1 mL dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 2000 (IHC, 50 µl/slide) |
Intended use | Research use only |
Tested species reactivity | bovine, human, rat |
Applications
Tested applications | Tested dilutions |
---|---|
Immunocytochemistry (ICC)/ Immunofluorescence (IF) | assay dependent |
Immunohistochemistry (IHC) - frozen | 1:10 - 1:100 |
Immunohistochemistry (IHC) - paraffin | 1:10 - 1:100 (microwave treatment recommended) |
Western Blot (WB) | assay dependent |
Background
Details
DG 3.10 recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide reacting: Mr 165 000 Desmoglein, "band 3" polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide.
Reactivity on cultured cell lines: A-431, MDBK.
Reference
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