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IHC of human oral mucosa using anti-Desmoglein 1 antibody (Cat. No. 651111) (courtesy of J.Heß, University Hospital Heidelberg) Zoom

IHC of human oral mucosa using anti-Desmoglein 1 antibody (Cat. No. 651111) (courtesy of J.Heß, University Hospital Heidelberg)

anti-desmoglein 1-4 sample set

The anti-desmoglein 1-4 sample set provides antibodies directed against Desmoglein 1, 2, 3 and 4 to evaluate their presence and status in IHC and WB. The set contains enough antibody to perform stainings on 6-12 sections per antibody.

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Cat. No.: 70033
Quantity:  600 µl each antibody

 

Cat. No. Product Name Reactivity Application
651111S anti-Desmoglein 1 mouse monoclonal, Dsg1-P124, supernatant, sample (ready-to-use) human IHC, WB
651119S anti-Desmoglein 2 mouse monoclonal, G129, supernatant, sample (ready-to-use) human, rat IHC, WB
651112S anti-Desmoglein 3 mouse monoclonal, Dsg-G194, supernatant, sample (ready-to-use) human IHC, WB
GP4S anti-Desmoglein 4 guinea pig polyclonal, serum, sample (ready-to-use) human, mouse, rat IHC

Price as configured: €147.00

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Specifications

Product description

Immunogen see individual antibody datasheet for information about specific immunogens
Storage short term at 2 – 8 °C; long term storage in aliquots at - 20 °C; avoid freeze/ thaw cycles
Number of experiments 6-12 sections per antibody
Note centrifuge prior to opening
Intended use research use only
Tested species reactivity see individual antibody datasheet

Applications

Tested applications Tested dilutions
Immunohistochemistry (IHC) - frozen ready-to-use
Immunohistochemistry (IHC) - paraffin ready-to-use (microwave treatment recommended)
Western Blot (WB) assay dependent (anti-Desmoglein 4, Cat. No. GP4S not tested)

Background

Details

As one of the stronger cell-to-cell adhesion types desmosomes allow cells to connect their intemediate filaments to those of neighboring cells. Therefore desmosomes can be found especially in cardiac muscle tissue, in bladder tissue and in epithelia, where the meachnical stress is quite high. As members of the cadherin super family, desmogleins and desmocollins thereby mediate adhesion at demosomes. Plakoglobin and the plakophilins on the other hand are members of the armadillo gene family and mediate the connection of the desmosome to desmoplakins that themselves mediate the connection to the cytoskeleton.

Mutations in these proteins or autoimmune reaction against them lead to severe diseases like arrhythmogenic right ventricular cardiomyopathy, Hailey-Hailey disease or striate palmoplantar keratoderma.

Furthermore the tissue-specific expression of desmosomal proteins is critical to tissue homeostasis. Missexpression of single components of desmosomes can lead to hyperproliferation and abnormal differentiation.

The anti-desmoglein 1-4 sample set provides antibodies directed against Desmoglein 1, 2, 3 and 4 to evaluate their presence and status in IHC and WB.  The set contains enough antibody to perform stainings on 6-12 sections per antibody.

Set content:
Cat. No. 651111S, anti-Desmoglein 1 mouse monoclonal, Dsg1-P124, supernatant, sample (ready-to-use), 600 µl
Cat. No. 651119S, anti-Desmoglein 2 mouse monoclonal, G129, supernatant, sample (ready-to-use), 600 µl
Cat. No. 651112S, anti-Desmoglein 3 mouse monoclonal, Dsg-G194, supernatant, sample (ready-to-use), 600 µl
Cat. No. GP4S, anti-Desmoglein 4 guinea pig polyclonal, serum, sample (ready-to-use), 600 µl

Reference

Reference

PublicationSpeciesApplicationAntibody
Nekrasova, O. et al. Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination. Nat.Commun. 9, 1053 (2018). human ICC-IF 651111
Vielmuth, F. et al. Keratins Regulate p38MAPK-Dependent Desmoglein Binding Properties in Pemphigus. Front.Immunol. 9, 528 (2018). mouse WB 651111
Bazzi, H. et al. Desmoglein 4 is expressed in highly differentiated keratinocytes and trichocytes in human epidermis and hair follicle. Differentiation 74, 129–140 (2006). human IHC (frozen) GP4
Waschke, J., Bruggeman, P., Baumgartner, W., Zillikens, D. & Drenckhahn, D. Pemphigus foliaceus IgG causes dissociation of desmoglein 1 – containing junctions without blocking desmoglein 1 transinteraction. J. Clin. Invest. 115, 3157–3165 (2005). human ICC-IF, dot blot 651111
Moll, I., Houdek, P., Schäfer, S., Nuber, U. & Moll, R. Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model. Arch. Dermatol. Res. 291, 437–46 (1999). human IHC (frozen)

651111, 651112, 651119

Kurzen, H. et al. Compositionally different desmosomes in the various compartments of the human hair follicle. Differentiation 63, 295–304 (1998). human WB, IHC (frozen, paraffin) 651111
Schäfer, S., Stumpp, S. & Franke, W. W. Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation 60, 99–108 (1996). human WB 651119

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