IHC of human oral mucosa (courtesy of J.Heß, University Hospital Heidelberg)

anti-Desmoglein 1 mouse monoclonal, Dsg1-P124, supernatant

The monoclonal anti-desmoglein 1 antibody stains basal and lower suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. Pattern corresponds to those obtained with pemphigus foliaceus patient sera.

Cat. No.: 651111
Quantity: 5 mL
No. of experiments: ≤ 5000 (IHC, 50 µl/slide)

Delivery Time: usually 1-7 working days

$290.00

Excl. VAT, excl. Shipping Cost

Qty:

Publication

Species

Application

Lewis, J. et al. The desmosome is a mesoscale lipid raft-like membrane domain. Mol.Biol.Cell. 30, 1390-1405 (2019)

human

IHC-IF (paraffin)

Nekrasova, O. et al. Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination. Nat.Commun. 9, 1053 (2018).

human

ICC-IF

Vielmuth, F. et al. Keratins Regulate p38MAPK-Dependent Desmoglein Binding Properties in Pemphigus. Front.Immunol. 9, 528 (2018).

mouse

Waschke, J., Bruggeman, P., Baumgartner, W., Zillikens, D. & Drenckhahn, D. Pemphigus foliaceus IgG causes dissociation of desmoglein 1 â containing junctions without blocking desmoglein 1 transinteraction. J. Clin. Invest. 115, 3157â3165 (2005).

human

ICC-IF
dot blot

Moll, I., Houdek, P., SchÃ¤fer, S., Nuber, U. & Moll, R. Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model. Arch. Dermatol. Res. 291, 437â46 (1999).

human

IHC (frozen)

Kurzen, H. et al. Compositionally different desmosomes in the various compartments of the human hair follicle. Differentiation 63, 295â304 (1998).

human

WB
IHC (frozen)
IHC (paraffin)

Alternative Products

Cat. No.	Product Name	Format	Quantity	Price
651111S	anti-Desmoglein 1 mouse monoclonal, Dsg1-P124	supernatant, sample (ready-to-use)	600 µL (ready-to-use)	$65.00 Excl. VAT, excl. Shipping Cost
70030	anti-Desmosome sample set 1		600 µl each antibody	$230.00
70033	anti-Desmoglein 1-4 sample set		600 µl each antibody	$184.00

Product description

Host	mouse
Antibody Type	monoclonal
Isotype	IgG2a
Clone	Dsg1-P124
Immunogen	recombinant human Desmoglein 1 (extracellular part EII, EIII and EIV)
Purification	hybridoma cell culture supernatant
Conjugate	unconjugated
Formulation	contains 0.09% sodium azide
Storage	short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
No. of experiments	≤ 5000 (IHC, 50 µl/slide)
Intended use	Research use only
Tested species reactivity	human

Applications

Tested applications	Tested dilutions
Immunohistochemistry (IHC) - frozen	undiluted - 1:50 (preincubation (directly after fixation) with 0.05 - 0.2% Triton X-100 for 5 – 10 min, depending on tissue type, recommended)
Immunohistochemistry (IHC) - paraffin	undiluted - 1:50 (preincubation with 0.001% trypsin and addition of 2% milk powder to the incubation media and microwave treatment recommended)
Western Blot (WB)	assay dependent

Background

Details

In immunoblots of cytoskeletal preparations from human skin the antibody reacts specifically with a 160 kD polypeptide typical for Desmoglein 1. In immunolocalization studies the mab shows typical staining of suprabasal layers of the epidermis and special regions of fetal anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus foliaceus patient sera. Cross-reaction with Desmogleins 2 and 3 was not detected.

Reference

Publication

Species

Application

Lewis, J. et al. The desmosome is a mesoscale lipid raft-like membrane domain. Mol.Biol.Cell. 30, 1390-1405 (2019)

human

IHC-IF (paraffin)

Nekrasova, O. et al. Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination. Nat.Commun. 9, 1053 (2018).

human

ICC-IF

Vielmuth, F. et al. Keratins Regulate p38MAPK-Dependent Desmoglein Binding Properties in Pemphigus. Front.Immunol. 9, 528 (2018).

mouse

human

ICC-IF
dot blot

human

IHC (frozen)

Kurzen, H. et al. Compositionally different desmosomes in the various compartments of the human hair follicle. Differentiation 63, 295â304 (1998).

human

WB
IHC (frozen)
IHC (paraffin)

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anti-Desmoglein 1 mouse monoclonal, Dsg1-P124, supernatant

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