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Alternative Products
Cat. No. | Product Name | Format | Quantity | Price |
---|---|---|---|---|
651111S |
anti-Desmoglein 1 mouse monoclonal, Dsg1-P124 |
supernatant, sample (ready-to-use) | 600 µL (ready-to-use) |
$65.00
Excl. VAT, excl. Shipping Cost
|
70030 |
anti-Desmosome sample set 1 |
600 µl each antibody |
$230.00
|
|
70033 |
anti-Desmoglein 1-4 sample set |
600 µl each antibody |
$184.00
|
Product description
Host | mouse |
---|---|
Antibody Type | monoclonal |
Isotype | IgG2a |
Clone | Dsg1-P124 |
Immunogen | recombinant human Desmoglein 1 (extracellular part EII, EIII and EIV) |
Purification | hybridoma cell culture supernatant |
Conjugate | unconjugated |
Formulation | contains 0.09% sodium azide |
Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
No. of experiments | ≤ 5000 (IHC, 50 µl/slide) |
Intended use | Research use only |
Tested species reactivity | human |
Applications
Tested applications | Tested dilutions |
---|---|
Immunohistochemistry (IHC) - frozen | undiluted - 1:50 (preincubation (directly after fixation) with 0.05 - 0.2% Triton X-100 for 5 – 10 min, depending on tissue type, recommended) |
Immunohistochemistry (IHC) - paraffin | undiluted - 1:50 (preincubation with 0.001% trypsin and addition of 2% milk powder to the incubation media and microwave treatment recommended) |
Western Blot (WB) | assay dependent |
Background
Details
In immunoblots of cytoskeletal preparations from human skin the antibody reacts specifically with a 160 kD polypeptide typical for Desmoglein 1. In immunolocalization studies the mab shows typical staining of suprabasal layers of the epidermis and special regions of fetal anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus foliaceus patient sera. Cross-reaction with Desmogleins 2 and 3 was not detected.
Reference
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Species
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human
IHC-IF (paraffin)
human
WB
IHC (frozen)
IHC (paraffin)
IHC (frozen)
IHC (paraffin)