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Alternative Products
| Cat. No. | Product Name | Format | Quantity | Price |
|---|---|---|---|---|
| 651110 |
anti-Desmoglein 1 mouse monoclonal, Dsg1-P23 |
supernatant | 5 mL |
$290.00
Excl. VAT, excl. Shipping Cost
|
Product description
| Host | mouse |
|---|---|
| Antibody Type | monoclonal |
| Isotype | IgG1 |
| Clone | Dsg1-P23 |
| Immunogen | recombinant human polypeptide Desmoglein 1 (extra-cellular part EII, EIII and EIV) |
| Purification | hybridoma cell culture supernatant concentrate |
| Conjugate | unconjugated |
| Formulation | contains 0.09% sodium azide |
| Storage | short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles |
| No. of experiments | ≤ 10000 (IHC, 50 µl/slide) |
| Note | centrifuge prior to opening |
| Intended use | Research use only |
| Tested species reactivity | human |
Applications
| Tested applications | Tested dilutions |
|---|---|
| Immunohistochemistry (IHC) - frozen | 1:1,000 (for better resolution preincubation (directly after fixation) with 0.05 - 0.2% Triton X-100 for 5 - 10 min, depending on tissue type, is recommended) |
| Immunohistochemistry (IHC) - paraffin | 1:1,000 (microwave treatment recommended; for better staining preincubation with 0.001% trypsin and addition of 2% milk powder to the incubation media is recommended) |
| Western Blot (WB) | assay dependent |
Background
Details
In immunoblots of cytoskeletal preparations from human skin the antibody reacts specifically with a 160 kD polypeptide typical for Desmoglein 1. In immunolocalization studies the mab shows typical staining of suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus foliaceus patient sera.
Cross-reaction with Desmogleins 2 and 3 was not detected.
Reference
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Species
Application
human
IHC (paraffin)
